GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:22791111
| Citation |
Ahn, DG, Choi, JK, Taylor, DR and Oh, JW (2012) Biochemical characterization of a recombinant SARS coronavirus nsp12 RNA-dependent RNA polymerase capable of copying viral RNA templates. Arch. Virol. 157:2095-104 |
|---|---|
| Abstract |
The severe acute respiratory syndrome coronavirus (SARS-CoV) RNA genome is replicated by a virus-encoded RNA replicase, the key component of which is the nonstructural protein 12 (nsp12). In this report, we describe the biochemical properties of a full-length recombinant SARS-CoV nsp12 RNA-dependent RNA polymerase (RdRp) capable of copying viral RNA templates. The purified SARS-CoV nsp12 showed both primer-dependent and primer-independent RNA synthesis activities using homopolymeric RNA templates. The RdRp activity was strictly dependent on Mn(2+). The nsp12 preferentially copied homopolymeric pyrimidine RNA templates in the absence of an added oligonucleotide primer. It was also able to initiate de novo RNA synthesis from the 3'-ends of both the plus- and minus-strand genome of SARS-CoV, using the 3'-terminal 36- and 37-nt RNA, respectively. The in vitro RdRp assay system established with a full-length nsp12 will be useful for understanding the mechanisms of coronavirus replication and for the development of anti-SARS-CoV agents. |
| Links |
PubMed Online version:10.1007/s00705-012-1404-x |
| Keywords |
3' Untranslated Regions; Cations, Divalent/metabolism; Coenzymes/metabolism; DNA Primers/genetics; Genome, Viral; Manganese/metabolism; RNA Replicase/genetics; RNA Replicase/metabolism; RNA, Viral/metabolism; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; SARS Virus/enzymology; SARS Virus/genetics |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
enables |
GO:0003968: RNA-directed 5'-3' RNA polymerase activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0001172: transcription, RNA-templated |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
Notes
See also
References
See Help:References for how to manage references in GONUTS.
