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PMID:22771832

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Citation

Song, Z, Liu, L, Yue, Y, Jiao, H, Lin, H, Sheikhahmadi, A, Everaert, N, Decuypere, E and Buyse, J (2012) Fasting alters protein expression of AMP-activated protein kinase in the hypothalamus of broiler chicks (Gallus gallus domesticus). Gen. Comp. Endocrinol. 178:546-55

Abstract

An experiment was conducted to investigate the effects of fasting and re-feeding on hypothalamic 5'-AMP-activated protein kinase (AMPK) levels and (an)orexigenic neuropeptides. Male Arbor Acres chicks (7-day-old, n=160) were allocated to four equal treatment groups: control chicks (fed ad libitum for 48 h, C48), chicks that were fasted for 48 h (F48), chicks that were first fasted for 48 h and then re-fed for 24h (F48C24), and chicks that were fed ad libitum for 72h (C72). Fasting for 48 h significantly (P<0.05) increased the ratio of phosphorylated AMPKα to total AMPKα and phosphorylated LKB1 to total LKB1, whereas re-feeding for 24h reduced these ratios to that of the ad libitum fed C72 chicks. The gene expressions of agouti-related peptide (AgRP), neuropeptide Y (NPY), melanocortin receptor 4, melanin-concentrating hormone, prepro-orexins and carnitine palmitoyltransferase-1 were significantly (P<0.05) increased in the fasted chicks relative to the ad libitum fed C48 group. The gene expression of pro-opiomelanocortin (POMC), as well as cocaine- and amphetamine-regulated transcript (CART) was not affected by the nutritional status. Fasting significantly (P<0.05) decreased the mRNA levels of fatty acid synthase (FAS) and sterol regulatory element binding protein-1 (SREBP-1). The results suggest that the LKB1/AMPK signal pathway is involved in the energy homeostasis of fasted chicks, and its possible role in feed intake regulation might be mediated by the AgRP/NPY rather than the POMC/CART pathway.

Links

PubMed Online version:10.1016/j.ygcen.2012.06.026

Keywords

AMP-Activated Protein Kinases/metabolism; Animals; Chickens/metabolism; Chickens/physiology; Fasting/physiology; Hypothalamus/metabolism; Male; Signal Transduction

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CHICK:INS

located_in

GO:0005615: extracellular space

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:STK11

involved_in

GO:0009267: cellular response to starvation

ECO:0000314: direct assay evidence used in manual assertion

P

  • occurs_in:(UBERON:0001898)

Seeded From UniProt

complete

CHICK:Q2LAI0

involved_in

GO:0009267: cellular response to starvation

ECO:0000314: direct assay evidence used in manual assertion

P

  • occurs_in:(UBERON:0001898)

Seeded From UniProt

complete

CHICK:Q2PUH1

involved_in

GO:0009267: cellular response to starvation

ECO:0000314: direct assay evidence used in manual assertion

P

  • occurs_in:(UBERON:0001898)

Seeded From UniProt

complete

CHICK:INS

part_of

GO:0005615: extracellular space

ECO:0000314: direct assay evidence used in manual assertion

C

part_of:(GO:0009267)

Seeded From UniProt

complete

CHICK:STK11

involved_in

GO:0009267: cellular response to starvation

ECO:0000314: direct assay evidence used in manual assertion

P

occurs_in:(UBERON:0001898)

Seeded From UniProt

complete

CHICK:Q2LAI0

involved_in

GO:0009267: cellular response to starvation

ECO:0000314: direct assay evidence used in manual assertion

P

occurs_in:(UBERON:0001898)

Seeded From UniProt

complete

CHICK:Q2PUH1

involved_in

GO:0009267: cellular response to starvation

ECO:0000314: direct assay evidence used in manual assertion

P

occurs_in:(UBERON:0001898)

Seeded From UniProt

complete

Notes

See also

References

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