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PMID:22760640

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Citation

Ding, Z, Wang, B, Moreno, I, Dupláková, N, Simon, S, Carraro, N, Reemmer, J, Pěnčík, A, Chen, X, Tejos, R, Skůpa, P, Pollmann, S, Mravec, J, Petrášek, J, Zažímalová, E, Honys, D, Rolčík, J, Murphy, A, Orellana, A, Geisler, M and Friml, J (2012) ER-localized auxin transporter PIN8 regulates auxin homeostasis and male gametophyte development in Arabidopsis. Nat Commun 3:941

Abstract

Auxin is a key coordinative signal required for many aspects of plant development and its levels are controlled by auxin metabolism and intercellular auxin transport. Here we find that a member of PIN auxin transporter family, PIN8 is expressed in male gametophyte of Arabidopsis thaliana and has a crucial role in pollen development and functionality. Ectopic expression in sporophytic tissues establishes a role of PIN8 in regulating auxin homoeostasis and metabolism. PIN8 co-localizes with PIN5 to the endoplasmic reticulum (ER) where it acts as an auxin transporter. Genetic analyses reveal an antagonistic action of PIN5 and PIN8 in the regulation of intracellular auxin homoeostasis and gametophyte as well as sporophyte development. Our results reveal a role of the auxin transport in male gametophyte development in which the distinct actions of ER-localized PIN transporters regulate cellular auxin homoeostasis and maintain the auxin levels optimal for pollen development and pollen tube growth.

Links

PubMed Online version:10.1038/ncomms1941

Keywords


Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:PIN5

located_in

GO:0005783: endoplasmic reticulum

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:PIN5

acts_upstream_of_or_within

GO:0009555: pollen development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:PIN5

acts_upstream_of_or_within

GO:0009555: pollen development

ECO:0000316: genetic interaction evidence used in manual assertion

AGI_LocusCode:AT5G15100

P

Seeded From UniProt

complete

ARATH:PIN8

located_in

GO:0005783: endoplasmic reticulum

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:PIN8

acts_upstream_of_or_within

GO:0010315: auxin efflux

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:PIN8

acts_upstream_of_or_within

GO:0010252: auxin homeostasis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:PIN8

acts_upstream_of_or_within

GO:0009555: pollen development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:PIN8

acts_upstream_of_or_within

GO:0009555: pollen development

ECO:0000316: genetic interaction evidence used in manual assertion

AGI_LocusCode:AT5G16530

P

Seeded From UniProt

complete


See also

References

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