GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:22750515

From GONUTS
Jump to: navigation, search
Citation

Gjørlund, MD, Nielsen, J, Pankratova, S, Li, S, Korshunova, I, Bock, E and Berezin, V (2012) Neuroligin-1 induces neurite outgrowth through interaction with neurexin-1β and activation of fibroblast growth factor receptor-1. FASEB J. 26:4174-86

Abstract

Neurexin-1 (NRXN1) and neuroligin-1 (NLGN1) are synaptic cell adhesion molecules that connect pre- and postsynaptic neurons at synapses and mediate signaling across the synapse, which modulates synaptic activity and determines the properties of neuronal networks. Defects in the genes encoding NLGN1 have been linked to cognitive diseases such as autism. The roles of both NRXN1 and NLGN1 during synaptogenesis have been studied extensively, but little is known about the role of these molecules in neuritogenesis, which eventually results in neuronal circuitry formation. The present study investigated the neuritogenic effect of NLGN1 in cultures of hippocampal neurons. Our results show that NLGN1, both in soluble and membrane-bound forms, induces neurite outgrowth that depends on the interaction with NRXN1β and on activation of fibroblast growth factor receptor-1. In addition, we demonstrate that a synthetic peptide, termed neurolide, which is modeled after a part of the binding interface of NLGN1 for NRXN1β, can bind to NRXN1β and mimic the biological properties of NLGN1 in vitro.

Links

PubMed Online version:10.1096/fj.11-202242

Keywords

Animals; Cell Adhesion Molecules; Cell Adhesion Molecules, Neuronal/genetics; Cell Adhesion Molecules, Neuronal/metabolism; Cell Line; Cells, Cultured; Hippocampus/cytology; Humans; Mice; Nerve Tissue Proteins/genetics; Nerve Tissue Proteins/metabolism; Neurites/metabolism; Neurons/metabolism; Rats; Receptor, Fibroblast Growth Factor, Type 1/genetics; Receptor, Fibroblast Growth Factor, Type 1/metabolism; Surface Plasmon Resonance

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:NRX1B

involved_in

GO:0031175: neuron projection development

ECO:0000316: genetic interaction evidence used in manual assertion

UniProtKB:Q62765

P

  • occurs_in:(CL:0002608)

Seeded From UniProt

complete

HUMAN:NRX1B

enables

GO:0005105: type 1 fibroblast growth factor receptor binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:NRX1B

involved_in

GO:0045743: positive regulation of fibroblast growth factor receptor signaling pathway

ECO:0000314: direct assay evidence used in manual assertion

P

  • has_input:(UniProtKB:P11362)

Seeded From UniProt

complete

RAT:NLGN1

involved_in

GO:0031175: neuron projection development

ECO:0000316: genetic interaction evidence used in manual assertion

UniProtKB:P58400

P

  • occurs_in:(CL:0002608)

Seeded From UniProt

complete

RAT:NLGN1

involved_in

GO:0140058: neuron projection arborization

ECO:0000316: genetic interaction evidence used in manual assertion

UniProtKB:P58400

P

  • occurs_in:(CL:0002608)

Seeded From UniProt

complete

RAT:NLGN1

involved_in

GO:0031175: neuron projection development

ECO:0000316: genetic interaction evidence used in manual assertion

UniProtKB:P58400

P

occurs_in:(CL:0002608)

Seeded From UniProt

complete

RAT:NLGN1

involved_in

GO:0140058: neuron projection arborization

ECO:0000316: genetic interaction evidence used in manual assertion

UniProtKB:P58400

P

occurs_in:(CL:0002608)

Seeded From UniProt

complete

HUMAN:NRX1B

involved_in

GO:0031175: neuron projection development

ECO:0000316: genetic interaction evidence used in manual assertion

UniProtKB:Q62765

P

occurs_in:(CL:0002608)

Seeded From UniProt

complete

HUMAN:NRX1B

enables

GO:0005105: type 1 fibroblast growth factor receptor binding

ECO:0000314: direct assay evidence used in manual assertion

F

positively_regulates:(GO:0018108)

Seeded From UniProt

complete

HUMAN:NRX1B

involved_in

GO:0045743: positive regulation of fibroblast growth factor receptor signaling pathway

ECO:0000314: direct assay evidence used in manual assertion

P

has_regulation_target:(UniProtKB:P11362)

Seeded From UniProt

complete

HUMAN:NLGN1

GO:0031175: neuron projection development

ECO:0000314:

P

"The ability of NLGN1 to induce neurite outgrowth was evaluated by incubating the neurons with increasing concentrations of recombinant NLGN1." (Page 4177) This was followed by fluorescence staining.

complete
CACAO 7162

HUMAN:NLGN1

involved_in

GO:0031175: neuron projection development

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.