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PMID:22731699

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Citation

Zhang, Y, Xu, YH, Yi, HY and Gong, JM (2012) Vacuolar membrane transporters OsVIT1 and OsVIT2 modulate iron translocation between flag leaves and seeds in rice. Plant J. 72:400-10

Abstract

The plant vacuole is an important organelle for storing excess iron (Fe), though its contribution to increasing the Fe content in staple foods remains largely unexplored. In this study we report the isolation and functional characterization of two rice genes OsVIT1 and OsVIT2, orthologs of the Arabidopsis VIT1. Transient expression of OsVIT1:EGFP and OsVIT2:EGFP protein fusions revealed that OsVIT1 and OsVIT2 are localized to the vacuolar membrane. Ectopic expression of OsVIT1 and OsVIT2 partially rescued the Fe(2+) - and Zn(2+) -sensitive phenotypes in yeast mutant Δccc1 and Δzrc1, and further increased vacuolar Fe(2+) , Zn(2+) and Mn(2+) accumulation. These data together suggest that OsVIT1 and OsVIT2 function to transport Fe(2+) , Zn(2+) and Mn(2+) across the tonoplast into vacuoles in yeast. In rice, OsVIT1 and OsVIT2 are highly expressed in flag leaf blade and sheath, respectively, and in contrast to OsVIT1, OsVIT2 is highly responsive to Fe treatments. Interestingly, functional disruption of OsVIT1 and OsVIT2 leads to increased Fe/Zn accumulation in rice seeds and a corresponding decrease in the source organ flag leaves, indicating an enhanced Fe/Zn translocation between source and sink organs, which might represent a novel strategy to biofortify Fe/Zn in staple foods.

Links

PubMed Online version:10.1111/j.1365-313X.2012.05088.x

Keywords

Amino Acid Sequence; Biological Transport; Gene Expression; Gene Expression Regulation, Plant/genetics; Intracellular Membranes/metabolism; Iron/metabolism; Manganese/metabolism; Membrane Transport Proteins/genetics; Membrane Transport Proteins/metabolism; Mutagenesis, Insertional; Organ Specificity; Oryza sativa/cytology; Oryza sativa/genetics; Oryza sativa/growth & development; Oryza sativa/metabolism; Phenotype; Phloem/cytology; Phloem/genetics; Phloem/growth & development; Phloem/metabolism; Plant Leaves/cytology; Plant Leaves/genetics; Plant Leaves/growth & development; Plant Leaves/metabolism; Plant Proteins/genetics; Plant Proteins/metabolism; Recombinant Fusion Proteins; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism; Seedling/cytology; Seedling/genetics; Seedling/growth & development; Seedling/metabolism; Seeds/cytology; Seeds/genetics; Seeds/growth & development; Seeds/metabolism; Sequence Alignment; Vacuoles/metabolism; Zinc/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ORYSJ:VIT2

involved_in

GO:0006880: intracellular sequestering of iron ion

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ORYSJ:VIT2

involved_in

GO:0006879: cellular iron ion homeostasis

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ORYSJ:VIT2

located_in

GO:0005774: vacuolar membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ORYSJ:VIT1

involved_in

GO:0006880: intracellular sequestering of iron ion

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ORYSJ:VIT1

involved_in

GO:0006879: cellular iron ion homeostasis

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ORYSJ:VIT1

located_in

GO:0005774: vacuolar membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ORYSJ:VIT11

enables

GO:0005381: iron ion transmembrane transporter activity

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete

ORYSJ:VIT11

GO:0005381: iron ion transmembrane transporter activity

ECO:0000315:

F

Fig 4, 5 & 6

complete
CACAO 8376

ORYSJ:VIT12

enables

GO:0005381: iron ion transmembrane transporter activity

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete

ORYSJ:VIT12

GO:0005381: iron ion transmembrane transporter activity

ECO:0000315:

F

Fig 4, 5 & 6

complete
CACAO 8377


See also

References

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