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PMID:22711701

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Citation

Llano, E, Herrán, Y, García-Tuñón, I, Gutiérrez-Caballero, C, de Álava, E, Barbero, JL, Schimenti, J, de Rooij, DG, Sánchez-Martín, M and Pendás, AM (2012) Meiotic cohesin complexes are essential for the formation of the axial element in mice. J. Cell Biol. 197:877-85

Abstract

Cohesin is a conserved multisubunit protein complex that participates in chromosome segregation, DNA damage repair, chromatin regulation, and synaptonemal complex (SC) formation. Yeast, but not mice, depleted of the cohesin subunit Rec8 are defective in the formation of the axial elements (AEs) of the SC, suggesting that, in mammals, this function is not conserved. In this paper, we show that spermatocytes from mice lacking the two meiosis-specific cohesin subunits RAD21L and REC8 were unable to initiate RAD51- but not DMC1-mediated double-strand break repair, were not able to assemble their AEs, and arrested as early as the leptotene stage of prophase I, demonstrating that cohesin plays an essential role in AE assembly that is conserved from yeast to mammals.

Links

PubMed PMC3384418 Online version:10.1083/jcb.201201100

Keywords

Animals; Cell Cycle Proteins/metabolism; Chromosomal Proteins, Non-Histone/metabolism; DNA Breaks, Double-Stranded; Male; Meiosis; Mice; Mice, Knockout; Nuclear Proteins/deficiency; Nuclear Proteins/metabolism; Phosphoproteins/deficiency; Phosphoproteins/metabolism; Protein Binding

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:RD21L

acts_upstream_of_or_within

GO:0000724: double-strand break repair via homologous recombination

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:1929645

P

  • occurs_in:(CL:0000017)

Seeded From UniProt

complete

MOUSE:RD21L

acts_upstream_of_or_within

GO:0007130: synaptonemal complex assembly

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:1929645

P

  • occurs_in:(CL:0000017)

Seeded From UniProt

complete

MOUSE:RD21L

acts_upstream_of_or_within

GO:0009566: fertilization

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:5292341

P

Seeded From UniProt

complete

MOUSE:RD21L

acts_upstream_of_or_within

GO:0072520: seminiferous tubule development

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:1929645

P

Seeded From UniProt

complete

MOUSE:STAG3

located_in

GO:0000800: lateral element

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(CL:0000017)

Seeded From UniProt

complete

MOUSE:H2AX

located_in

GO:0000794: condensed nuclear chromosome

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(CL:0000017)

Seeded From UniProt

complete

MOUSE:SYCP3

located_in

GO:0000800: lateral element

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(CL:0000017)

Seeded From UniProt

complete

MOUSE:DMC1

located_in

GO:0000794: condensed nuclear chromosome

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(CL:0000017)

Seeded From UniProt

complete

MOUSE:SYCP1

located_in

GO:0000802: transverse filament

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(CL:0000017)

Seeded From UniProt

complete

MOUSE:REC8

acts_upstream_of_or_within

GO:0000724: double-strand break repair via homologous recombination

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:3652039

P

  • occurs_in:(CL:0000017)

Seeded From UniProt

complete

MOUSE:REC8

acts_upstream_of_or_within

GO:0007130: synaptonemal complex assembly

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:3652039

P

  • occurs_in:(CL:0000017)

Seeded From UniProt

complete

MOUSE:REC8

acts_upstream_of_or_within

GO:0009566: fertilization

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:3512754

P

Seeded From UniProt

complete

MOUSE:REC8

acts_upstream_of_or_within

GO:0072520: seminiferous tubule development

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:3652039

P

Seeded From UniProt

complete

MOUSE:RFA1

located_in

GO:0000800: lateral element

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(CL:0000017)

Seeded From UniProt

complete

MOUSE:SMC1B

located_in

GO:0000800: lateral element

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(CL:0000017)

Seeded From UniProt

complete

MOUSE:SYCP2

located_in

GO:0000800: lateral element

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(CL:0000017)

Seeded From UniProt

complete

MOUSE:SMC3

located_in

GO:0000800: lateral element

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(CL:0000017)

Seeded From UniProt

complete

MOUSE:SYCP1

part_of

GO:0000802: transverse filament

ECO:0000314: direct assay evidence used in manual assertion

C

part_of:(CL:0000017)

Seeded From UniProt

complete

MOUSE:H2AX

part_of

GO:0000794: condensed nuclear chromosome

ECO:0000314: direct assay evidence used in manual assertion

C

part_of:(CL:0000017)

Seeded From UniProt

complete

Notes

See also

References

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