GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:22705394

From GONUTS
Jump to: navigation, search
Citation

Burgo, A, Proux-Gillardeaux, V, Sotirakis, E, Bun, P, Casano, A, Verraes, A, Liem, RK, Formstecher, E, Coppey-Moisan, M and Galli, T (2012) A molecular network for the transport of the TI-VAMP/VAMP7 vesicles from cell center to periphery. Dev. Cell 23:166-80

Abstract

The compartmental organization of eukaryotic cells is maintained dynamically by vesicular trafficking. SNARE proteins play a crucial role in intracellular membrane fusion and need to be targeted to their proper donor or acceptor membrane. The molecular mechanisms that allow for the secretory vesicles carrying the v-SNARE TI-VAMP/VAMP7 to leave the cell center, load onto microtubules, and reach the periphery to mediate exocytosis are largely unknown. Here, we show that the TI-VAMP/VAMP7 partner Varp, a Rab21 guanine nucleotide exchange factor, interacts with GolginA4 and the kinesin 1 Kif5A. Activated Rab21-GTP in turn binds to MACF1, an actin and microtubule regulator, which is itself a partner of GolginA4. These components are required for directed movement of TI-VAMP/VAMP7 vesicles from the cell center to the cell periphery. The molecular mechanisms uncovered here suggest an integrated view of the transport of vesicles carrying a specific v-SNARE toward the cell surface.

Links

PubMed Online version:10.1016/j.devcel.2012.04.019

Keywords

Animals; COS Cells; Cercopithecus aethiops; Golgi Apparatus/metabolism; Growth Cones/drug effects; Growth Cones/metabolism; HeLa Cells; Humans; Kinesin/genetics; Kinesin/metabolism; Nocodazole/pharmacology; Protein Transport/drug effects; Protein Transport/physiology; R-SNARE Proteins/metabolism; RNA, Small Interfering/genetics; Tubulin Modulators/pharmacology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:KIF5A

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q96NW4

F

Seeded From UniProt

complete

HUMAN:KIF5A

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q96NW4

F

Seeded From UniProt

complete

HUMAN:GOGA4

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q96NW4

F

Seeded From UniProt

complete

RAT:RAB21

involved_in

GO:0008089: anterograde axonal transport

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:ANR27

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P33175

F

Seeded From UniProt

complete

HUMAN:ANR27

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q12840

F

Seeded From UniProt

complete

HUMAN:ANR27

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q13439

F

Seeded From UniProt

complete

HUMAN:RAB21

involved_in

GO:0017157: regulation of exocytosis

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:RAB21

involved_in

GO:0008089: anterograde axonal transport

ECO:0006062: wide-field fluorescence microscopy evidence used in manual assertion

P

  • occurs_in:(UBERON:0002421)

Seeded From UniProt

complete

HUMAN:RAB21

involved_in

GO:0008089: anterograde axonal transport

ECO:0006063: over expression analysis evidence used in manual assertion

P

  • occurs_in:(UBERON:0002421)

Seeded From UniProt

complete

HUMAN:MACF1

involved_in

GO:0045773: positive regulation of axon extension

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:GOGA4

involved_in

GO:0045773: positive regulation of axon extension

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:GOGA4

involved_in

GO:0043001: Golgi to plasma membrane protein transport

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

RAT:VAMP7

involved_in

GO:0047496: vesicle transport along microtubule

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

RAT:VAMP7

involved_in

GO:0006887: exocytosis

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.