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PMID:22688510

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Citation

Lewis, PW, Sahoo, D, Geng, C, Bell, M, Lipsick, JS and Botchan, MR (2012) Drosophila lin-52 acts in opposition to repressive components of the Myb-MuvB/dREAM complex. Mol. Cell. Biol. 32:3218-27

Abstract

The Drosophila melanogaster Myb-MuvB/dREAM complex (MMB/dREAM) participates in both the activation and repression of developmentally regulated genes and origins of DNA replication. Mutants in MMB subunits exhibit diverse phenotypes, including lethality, eye defects, reduced fecundity, and sterility. Here, we used P-element excision to generate mutations in lin-52, which encodes the smallest subunit of the MMB/dREAM complex. lin-52 is required for viability, as null mutants die prior to pupariation. The generation of somatic and germ line mutant clones indicates that lin-52 is required for adult eye development and for early embryogenesis via maternal effects. Interestingly, the maternal-effect embryonic lethality, larval lethality, and adult eye defects could be suppressed by mutations in other subunits of the MMB/dREAM complex. These results suggest that a partial MMB/dREAM complex is responsible for the lethality and eye defects of lin-52 mutants. Furthermore, these findings support a model in which the Lin-52 and Myb proteins counteract the repressive activities of the other members of the MMB/dREAM complex at specific genomic loci in a developmentally controlled manner.

Links

PubMed PMC3434544 Online version:10.1128/MCB.00432-12

Keywords

Animals; Cell Cycle Proteins/genetics; Cell Cycle Proteins/metabolism; Chromatography, Gel; Crosses, Genetic; DNA Replication; Drosophila Proteins/genetics; Drosophila Proteins/metabolism; Drosophila melanogaster; E2F2 Transcription Factor/genetics; E2F2 Transcription Factor/metabolism; Female; Gene Expression Regulation, Developmental; Male; Microscopy, Fluorescence/methods; Models, Biological; Models, Genetic; Mutation; Photoreceptor Cells, Invertebrate/metabolism; Proto-Oncogene Proteins c-myb/genetics; Proto-Oncogene Proteins c-myb/metabolism; RNA Interference; Transcription Factors/genetics; Transcription Factors/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:Q9W482

located_in

GO:0000791: euchromatin

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:Q9W482

involved_in

GO:0048749: compound eye development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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