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PMID:22634526

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Citation

Sampson, CJ, Valanne, S, Fauvarque, MO, Hultmark, D, Rämet, M and Williams, MJ (2012) The RhoGEF Zizimin-related acts in the Drosophila cellular immune response via the Rho GTPases Rac2 and Cdc42. Dev. Comp. Immunol. 38:160-8

Abstract

Zizimin-related (Zir), a Rho guanine nucleotide exchange factor (RhoGEF) homologous to the mammalian Dock-C/Zizimin-related family, was identified in a screen to find new genes involved in the Drosophila melanogaster cellular immune response against eggs from the parasitoid wasp Leptopilina boulardi. RhoGEFs activate Rho-family GTPases, which are known to be central regulators of cell migration, spreading and polarity. When a parasitoid wasp is recognized as foreign, multiple layers of circulating immunosurveillance cells (haemocytes) should attach to the egg. In Zir mutants this process is disrupted and lamellocytes, a haemocyte subtype, fail to properly encapsulate the wasp egg. Furthermore, macrophage-like plasmatocytes exhibit a strong reduction in their ability to phagocytise Escherichia coli and Staphylococcus aureus bacteria. During encapsulation and phagocytosis Zir genetically interacts with two Rho-family GTPases, Rac2 and Cdc42. Finally, Zir is dispensable for the humoral immune response against bacteria. We propose that Zir is necessary to activate the Rho-family GTPases Rac2 and Cdc42 during the Drosophila cellular immune response.

Links

PubMed Online version:10.1016/j.dci.2012.05.004

Keywords

Animals; Drosophila Proteins/metabolism; Drosophila melanogaster/genetics; Drosophila melanogaster/growth & development; Drosophila melanogaster/immunology; Drosophila melanogaster/metabolism; GTP-Binding Proteins/metabolism; Guanine Nucleotide Exchange Factors/metabolism; Humans; Immunity, Cellular; Phagocytosis; Phylogeny; rac GTP-Binding Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:RAC1

involved_in

GO:0035010: encapsulation of foreign target

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:CDC42

involved_in

GO:0007266: Rho protein signal transduction

ECO:0000316: genetic interaction evidence used in manual assertion

FB:FBgn0031216

P

Seeded From UniProt

complete

DROME:CDC42

involved_in

GO:0006909: phagocytosis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:CDC42

involved_in

GO:0035010: encapsulation of foreign target

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:RAC2

involved_in

GO:0007266: Rho protein signal transduction

ECO:0000316: genetic interaction evidence used in manual assertion

FB:FBgn0031216

P

Seeded From UniProt

complete

DROME:RAC2

involved_in

GO:0006909: phagocytosis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:RAC2

involved_in

GO:0035010: encapsulation of foreign target

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9VPI9

involved_in

GO:0006909: phagocytosis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9VPI9

enables

GO:0005085: guanyl-nucleotide exchange factor activity

ECO:0000316: genetic interaction evidence used in manual assertion

FB:FBgn0010341,FB:FBgn0014011

F

Seeded From UniProt

complete

DROME:Q9VPI9

involved_in

GO:0035023: regulation of Rho protein signal transduction

ECO:0000316: genetic interaction evidence used in manual assertion

FB:FBgn0010341,FB:FBgn0014011

P

Seeded From UniProt

complete

DROME:Q9VPI9

involved_in

GO:0035010: encapsulation of foreign target

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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