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PMID:22617193

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Citation

Kwok, AH, Wang, Y and Leung, FC (2012) Molecular characterization of prostaglandin F receptor (FP) and E receptor subtype 1 (EP(1)) in zebrafish. Gen. Comp. Endocrinol. 178:216-26

Abstract

Prostaglandins E (PGE) and F (PGF) mediate diverse physiological functions via their cell surface receptors - prostaglandin E receptor (EP) subtypes 1, 2, 3 and 4 (EP(1); EP(2); EP(3); EP(4)) and F receptor (FP). In teleost fishes, PGE was implicated in gill epithelium ion transport, while both PGE and PGF were involved in oocyte maturation, follicular rupture and coordination of reproductive behaviors. However, little is known about the mechanisms behind their actions. In present study, we first identified the full-length ORF cDNA clones of three zebrafish prostaglandin E receptor subtype 1 (zEP(1)) isoforms - zEP(1a), zEP(1b) and zEP(1c) - and FP (zFP) from adult ovary. RT-PCR showed that zEP(1a), zEP(1b) and zFP are widely expressed in adult tissues, while zEP(1c) mRNA expression is mainly confined in brain and kidney. Using a pGL3-NFAT-RE luciferase reporter system, both zEP(1a) and zEP(1b) expressed in DF-1 cells were shown to be activated by PGE(2) potently while zEP(1c) and zFP were activated by PGF(2a) effectively, suggesting that the four receptors are functionally coupled to intracellular Ca(2+)-signaling pathway. Furthermore, EP1a and EP1b, but not EP1c were suggested to couple to cAMP-PKA signaling pathway using a pGL3-CRE luciferase reporter assay. Although zEP(1c) might originate as a paralog to zEP(1a) and zEP(1b), its functional coupling to PGF(2α) instead of PGE(2) suggested that zEP(1) isoforms might have sub-functionalized in their ligand binding and G protein coupling specificity, in addition to differential tissue distribution. Characterization of these receptors undoubtedly furthered our understanding on the diverse yet highly target-specific responses of prostaglandins in teleosts.

Links

PubMed Online version:10.1016/j.ygcen.2012.05.002

Keywords


Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DANRE:A0A2R8Q772

enables

GO:0004958: prostaglandin F receptor activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

DANRE:D0EXE2

enables

GO:0004957: prostaglandin E receptor activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

DANRE:D0EXE3

enables

GO:0004957: prostaglandin E receptor activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

DANRE:D0EXE4

enables

GO:0004958: prostaglandin F receptor activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

DANRE:D7R7W5

enables

GO:0004958: prostaglandin F receptor activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

DANRE:E7F7H2

enables

GO:0004958: prostaglandin F receptor activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

DANRE:F1QIS8

enables

GO:0004957: prostaglandin E receptor activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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