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PMID:22613836

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Citation

Gebert, M, Schrempp, SG, Mehnert, CS, Heißwolf, AK, Oeljeklaus, S, Ieva, R, Bohnert, M, von der Malsburg, K, Wiese, S, Kleinschroth, T, Hunte, C, Meyer, HE, Haferkamp, I, Guiard, B, Warscheid, B, Pfanner, N and van der Laan, M (2012) Mgr2 promotes coupling of the mitochondrial presequence translocase to partner complexes. J. Cell Biol. 197:595-604

Abstract

Many mitochondrial proteins are synthesized with N-terminal presequences in the cytosol. The presequence translocase of the inner mitochondrial membrane (TIM23) translocates preproteins into and across the membrane and associates with the matrix-localized import motor. The TIM23 complex consists of three core components and Tim21, which interacts with the translocase of the outer membrane (TOM) and the respiratory chain. We have identified a new subunit of the TIM23 complex, the inner membrane protein Mgr2. Mitochondria lacking Mgr2 were deficient in the Tim21-containing sorting form of the TIM23 complex. Mgr2 was required for binding of Tim21 to TIM23(CORE), revealing a binding chain of TIM23(CORE)-Mgr2/Tim21-respiratory chain. Mgr2-deficient yeast cells were defective in growth at elevated temperature, and the mitochondria were impaired in TOM-TIM23 coupling and the import of presequence-carrying preproteins. We conclude that Mgr2 is a coupling factor of the presequence translocase crucial for cell growth at elevated temperature and for efficient protein import.

Links

PubMed PMC3365495 Online version:10.1083/jcb.201110047

Keywords

Cell Proliferation; Membrane Proteins/metabolism; Membrane Transport Proteins/chemistry; Membrane Transport Proteins/metabolism; Mitochondrial Membrane Transport Proteins/chemistry; Mitochondrial Membrane Transport Proteins/metabolism; Multiprotein Complexes/chemistry; Multiprotein Complexes/metabolism; Protein Subunits/metabolism; Protein Transport; Saccharomyces cerevisiae/cytology; Saccharomyces cerevisiae/growth & development; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/chemistry; Saccharomyces cerevisiae Proteins/metabolism; Temperature

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:MGR2

involved_in

GO:0030150: protein import into mitochondrial matrix

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:MGR2

part_of

GO:0005744: TIM23 mitochondrial import inner membrane translocase complex

ECO:0000353: physical interaction evidence used in manual assertion

SGD:S000003265

C

Seeded From UniProt

complete


See also

References

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