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PMID:22588722

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Citation

Kim, J and Rose, MD (2012) A mechanism for the coordination of proliferation and differentiation by spatial regulation of Fus2p in budding yeast. Genes Dev. 26:1110-21

Abstract

Yeast cells induce the genes required for mating prior to the completion of mitosis. To ensure proper cell cycle progression prior to mating differentiation, a key cytoplasmic regulator of cell fusion, Fus2p, is sequestered in the nucleus by cyclin-dependent kinase (Cdk). In response to pheromone signaling, the mitogen-activated protein kinase Fus3p phosphorylates Ser 84 in Fus2p to drive nuclear export. We found that Fus3p becomes active and phosphorylates S84 as early as S phase, raising the question of how Cdk prevents inappropriate activation of Fus2p. Countering Fus3p, Cdk and a p21-activated kinase, Cla4p, maintain Fus2p's nuclear localization by phosphorylating Ser 67, which drives nuclear import and inhibits nuclear export. When Cdk and Cla4p activities drop after cell division, Fus3p promotes Fus2p export both via S84 phosphorylation and by down-regulating S67 phosphorylation. Thus, potential premature activation of Fus2p in mitosis is prevented by cell cycle-dependent phosphorylation that overrides the mating pheromone-induced phosphorylation that drives nuclear export.

Links

PubMed PMC3360565 Online version:10.1101/gad.187260.112

Keywords

Active Transport, Cell Nucleus; Amino Acid Sequence; Cell Differentiation; Cell Nucleus/metabolism; Cell Proliferation; Cyclin-Dependent Kinases/metabolism; Cytoskeletal Proteins/genetics; Cytoskeletal Proteins/metabolism; Karyopherins/metabolism; Membrane Proteins/genetics; Membrane Proteins/metabolism; Mitogens/pharmacology; Mitosis; Molecular Sequence Data; Nuclear Localization Signals/genetics; Nuclear Localization Signals/metabolism; Phosphorylation; Protein-Serine-Threonine Kinases/metabolism; Receptors, Cytoplasmic and Nuclear/metabolism; Saccharomyces cerevisiae/cytology; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae/physiology; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; Serine/genetics; Serine/metabolism; p21-Activated Kinases/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:FUS3

involved_in

GO:0006468: protein phosphorylation

ECO:0000314: direct assay evidence used in manual assertion

P

  • has_input:(SGD:S000004845)

Seeded From UniProt

complete

YEAST:FUS3

involved_in

GO:0046827: positive regulation of protein export from nucleus

ECO:0000315: mutant phenotype evidence used in manual assertion

P

  • has_input:(SGD:S000004845)

Seeded From UniProt

complete

YEAST:CLA4

involved_in

GO:0006468: protein phosphorylation

ECO:0000314: direct assay evidence used in manual assertion

P

  • has_input:(UniProtKB:Q05670)

Seeded From UniProt

complete

YEAST:CLA4

enables

GO:0004674: protein serine/threonine kinase activity

ECO:0000314: direct assay evidence used in manual assertion

F

  • has_input:(UniProtKB:Q05670)
  • happens_during:(GO:0000278)

Seeded From UniProt

complete

YEAST:CLA4

involved_in

GO:0042307: positive regulation of protein import into nucleus

ECO:0000315: mutant phenotype evidence used in manual assertion

P

  • has_input:(UniProtKB:Q05670)

Seeded From UniProt

complete

YEAST:CLA4

enables

GO:0004674: protein serine/threonine kinase activity

ECO:0000314: direct assay evidence used in manual assertion

F

  • has_direct_input:(UniProtKB:Q05670)
  • happens_during:(GO:0000278)

Seeded From UniProt

complete

YEAST:CLA4

involved_in

GO:0006468: protein phosphorylation

ECO:0000314: direct assay evidence used in manual assertion

P

has_direct_input:(UniProtKB:Q05670)

Seeded From UniProt

complete

YEAST:CLA4

involved_in

GO:0042307: positive regulation of protein import into nucleus

ECO:0000315: mutant phenotype evidence used in manual assertion

P

has_direct_input:(UniProtKB:Q05670)

Seeded From UniProt

complete

YEAST:FUS3

involved_in

GO:0006468: protein phosphorylation

ECO:0000314: direct assay evidence used in manual assertion

P

has_direct_input:(UniProtKB:Q05670)

Seeded From UniProt

complete

YEAST:FUS3

involved_in

GO:0046827: positive regulation of protein export from nucleus

ECO:0000315: mutant phenotype evidence used in manual assertion

P

has_direct_input:(UniProtKB:Q05670)

Seeded From UniProt

complete


See also

References

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