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PMID:22582791

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Citation

De Jonge, N, Simic, M, Buts, L, Haesaerts, S, Roelants, K, Garcia-Pino, A, Sterckx, Y, De Greve, H, Lah, J and Loris, R (2012) Alternative interactions define gyrase specificity in the CcdB family. Mol. Microbiol. 84:965-78

Abstract

Toxin-antitoxin (TA) modules are small operons associated with stress response of bacteria. F-plasmid CcdB(F) was the first TA toxin for which its target, gyrase, was identified. Plasmidic and chromosomal CcdBs belong to distinct families. Conserved residues crucial for gyrase poisoning activity of plasmidic CcdBs are not conserved among these families. Here we show that the chromosomal CcdB(Vfi) from Vibrio fischeri is an active gyrase poison that interacts with its target via an alternative energetic mechanism. Changes in the GyrA14-binding surface of the Vibrio and F-plasmid CcdB family members illustrate neutral drift where alternative interactions can be used to achieve the same functionality. Differences in affinity between V. fischeri and F-plasmid CcdB for gyrase and their corresponding CcdA antitoxin possibly reflect distinct roles for TA modules located on plasmids and chromosomes.

Links

PubMed Online version:10.1111/j.1365-2958.2012.08069.x

Keywords

Aliivibrio fischeri/enzymology; Aliivibrio fischeri/genetics; Aliivibrio fischeri/metabolism; Amino Acid Substitution; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; DNA Gyrase/antagonists & inhibitors; Models, Molecular; Mutagenesis, Site-Directed; Mutant Proteins/genetics; Mutant Proteins/metabolism; Plasmids; Protein Conformation; Protein Interaction Mapping; Substrate Specificity

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

VIBFI:Q84B82

GO:0008657: DNA topoisomerase (ATP-hydrolyzing) inhibitor activity

ECO:0000314:

F

Fig 2. Shows CcdBvfi as a stabalizer of inhibitor activity. It is combined with different concentrations of CcdAvfi, and DNA gyrase to show the inhibition of DNA gyrase activity when CcdAvfi and CcdAvfi are unable to bind in a one to one ratio.

complete
CACAO 8710

See also

References

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