GONUTS has been updated to MW1.29.2. Most things seem to be working but be sure to report problems.

It is now the 6th OPEN Period for CACAO Spring 2018! It will end on Monday April 23, 2018 at 05:59 pm CDT
This is your chance to make annotations OR challenge other team's annotations. You may also DEFEND or suggest improvements to your own annotations IF they have been challenged. Please note, although we ENCOURAGE challenges, an excess of identical challenges that do not appear to be applicable to the annotation or well thought out will be considered spam and ignored.

It is now the 4th OPEN Period for CACAO Phage Hunters 2018! It will end on Sunday April 22, 2018 at 11:59 pm CDT
This is your chance to make annotations OR challenge other team's annotations. You may also DEFEND or suggest improvements to your own annotations IF they have been challenged. Please note, although we ENCOURAGE challenges, an excess of identical challenges that do not appear to be applicable to the annotation or well thought out will be considered spam and ignored.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:22547825

From GONUTS
Jump to: navigation, search
Citation

Fichelson, P, Brigui, A and Pichaud, F (2012) Orthodenticle and Kruppel homolog 1 regulate Drosophila photoreceptor maturation. Proc. Natl. Acad. Sci. U.S.A. 109:7893-8

Abstract

Neurons present a wide variety of morphologies that are associated with their specialized functions. However, to date very few pathways and factors regulating neuronal maturation, including morphogenesis, have been identified. To address this issue we make use here of the genetically amenable developing fly photoreceptor (PR). Whereas this sensory neuron is specified early during retinal development, its maturation spans several days. During this time, this neuron acquires specialized membrane domains while undergoing extensive polarity remodeling. In this study, we identify a pathway in which the conserved homeobox protein Orthodenticle (Otd) acts together with the ecdysone receptor (EcR) to directly repress the expression of the transcription factor (TF) Kruppel homolog 1 (Kr-h1). We demonstrate that this pathway is not required to promote neuronal specification but is crucial to regulate PR maturation. PR maturation includes the remodeling of the cell's epithelial features and associated apical membrane morphogenesis. Furthermore, we show that hormonal control coordinates PR differentiation and morphogenesis with overall development. This study demonstrates that during PR differentiation, transient repression of Kr-h1 represents a key step regulating neuronal maturation. Down-regulation of Kr-h1 expression has been previously associated with instances of neuronal remodeling in the fly brain. We therefore conclude that repression of this transcription factor represents a key step, enabling remodeling and maturation in a wide variety of neurons.

Links

PubMed PMC3356647 Online version:10.1073/pnas.1120276109

Keywords

Animals; Chromatin Immunoprecipitation; Cloning, Molecular; DNA Primers/genetics; Drosophila/growth & development; Drosophila Proteins/metabolism; Gene Expression Regulation, Developmental/physiology; Homeodomain Proteins/metabolism; Immunohistochemistry; Kruppel-Like Transcription Factors/metabolism; Larva/growth & development; Larva/metabolism; Microarray Analysis; Microscopy, Confocal; Microscopy, Electron, Transmission; Photoreceptor Cells, Invertebrate/physiology; Receptors, Steroid/metabolism; Retina/ultrastructure; Reverse Transcriptase Polymerase Chain Reaction

Significance

Annotations

Gene product Qualifier GO ID GO term name Evidence Code with/from Aspect Notes Status

FB:oc

GO:0045315

positive regulation of compound eye photoreceptor development

IMP: Inferred from Mutant Phenotype

P

From FB


FB:Kr-h1

GO:0045316

negative regulation of compound eye photoreceptor development

IMP: Inferred from Mutant Phenotype

P

From FB


DROME:HMOC

GO:0045315

positive regulation of compound eye photoreceptor development

IMP: Inferred from Mutant Phenotype

P

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.