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PMID:22540282

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Citation

Riewe, D, Koohi, M, Lisec, J, Pfeiffer, M, Lippmann, R, Schmeichel, J, Willmitzer, L and Altmann, T (2012) A tyrosine aminotransferase involved in tocopherol synthesis in Arabidopsis. Plant J. 71:850-859

Abstract

The metabolic function of the predicted Arabidopsis tyrosine aminotransferase (TAT) encoded by the At5g53970 gene was studied using two independent knock-out mutants. Gas chromatography-mass spectrometry based metabolic profiling revealed a specific increase in tyrosine levels, supporting the proposed function of At5g53970 as a tyrosine-specific aminotransferase not involved in tyrosine biosynthesis, but rather in utilization of tyrosine for other metabolic pathways. The TAT activity of the At5g53970-encoded protein was verified by complementation of the Escherichia coli tyrosine auxotrophic mutant DL39, and in vitro activity of recombinantly expressed and purified At5g53970 was found to be specific for tyrosine. To investigate the physiological role of At5g53970, the consequences of reduction in tyrosine utilization on metabolic pathways having tyrosine as a substrate were analysed. We found that tocopherols were substantially reduced in the mutants and we conclude that At5g53970 encodes a TAT important for the synthesis of tocopherols in Arabidopsis.

Links

PubMed Online version:10.1111/j.1365-313X.2012.05035.x

Keywords


Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:TAT2

acts_upstream_of_or_within

GO:0010189: vitamin E biosynthetic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:TAT2

enables

GO:0004838: L-tyrosine:2-oxoglutarate aminotransferase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:TAT2

enables

GO:0004838: L-tyrosine:2-oxoglutarate aminotransferase activity

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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