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PMID:22523205

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Citation

Laohavisit, A, Shang, Z, Rubio, L, Cuin, TA, Véry, AA, Wang, A, Mortimer, JC, Macpherson, N, Coxon, KM, Battey, NH, Brownlee, C, Park, OK, Sentenac, H, Shabala, S, Webb, AA and Davies, JM (2012) Arabidopsis annexin1 mediates the radical-activated plasma membrane Ca²+- and K+-permeable conductance in root cells. Plant Cell 24:1522-33

Abstract

Plant cell growth and stress signaling require Ca²⁺ influx through plasma membrane transport proteins that are regulated by reactive oxygen species. In root cell growth, adaptation to salinity stress, and stomatal closure, such proteins operate downstream of the plasma membrane NADPH oxidases that produce extracellular superoxide anion, a reactive oxygen species that is readily converted to extracellular hydrogen peroxide and hydroxyl radicals, OH•. In root cells, extracellular OH• activates a plasma membrane Ca²⁺-permeable conductance that permits Ca²⁺ influx. In Arabidopsis thaliana, distribution of this conductance resembles that of annexin1 (ANN1). Annexins are membrane binding proteins that can form Ca²⁺-permeable conductances in vitro. Here, the Arabidopsis loss-of-function mutant for annexin1 (Atann1) was found to lack the root hair and epidermal OH•-activated Ca²⁺- and K⁺-permeable conductance. This manifests in both impaired root cell growth and ability to elevate root cell cytosolic free Ca²⁺ in response to OH•. An OH•-activated Ca²⁺ conductance is reconstituted by recombinant ANN1 in planar lipid bilayers. ANN1 therefore presents as a novel Ca²⁺-permeable transporter providing a molecular link between reactive oxygen species and cytosolic Ca²⁺ in plants.

Links

PubMed PMC3398561 Online version:10.1105/tpc.112.097881

Keywords

Annexin A1/metabolism; Arabidopsis/cytology; Arabidopsis/drug effects; Arabidopsis/physiology; Arabidopsis Proteins/metabolism; Calcium/metabolism; Calcium Channels/metabolism; Cell Membrane/drug effects; Cell Membrane/metabolism; Cell Membrane Permeability/drug effects; Diffusion/drug effects; Hydroxyl Radical/pharmacology; Ion Channel Gating/drug effects; Lipid Bilayers/metabolism; Plant Cells/drug effects; Plant Cells/metabolism; Plant Epidermis/drug effects; Plant Epidermis/metabolism; Plant Roots/cytology; Plant Roots/drug effects; Plant Roots/physiology; Potassium/metabolism; Protoplasts/drug effects; Protoplasts/metabolism; Recombinant Proteins/isolation & purification; Shaker Superfamily of Potassium Channels/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:ANXD1

acts_upstream_of_or_within

GO:0070588: calcium ion transmembrane transport

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:ANXD1

acts_upstream_of_or_within

GO:0097623: potassium ion export across plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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