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PMID:22499592

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Citation

Kim, YJ, Bao, H, Bonanno, L, Zhang, B and Serpe, M (2012) Drosophila Neto is essential for clustering glutamate receptors at the neuromuscular junction. Genes Dev. 26:974-87

Abstract

Neurotransmitter receptor recruitment at postsynaptic specializations is key in synaptogenesis, since this step confers functionality to the nascent synapse. The Drosophila neuromuscular junction (NMJ) is a glutamatergic synapse, similar in composition and function to mammalian central synapses. Various mechanisms regulating the extent of postsynaptic ionotropic glutamate receptor (iGluR) clustering have been described, but none are known to be essential for the initial localization and clustering of iGluRs at postsynaptic densities (PSDs). We identified and characterized the Drosophila neto (neuropilin and tolloid-like) as an essential gene required for clustering of iGluRs at the NMJ. Neto colocalizes with the iGluRs at the PSDs in puncta juxtaposing the active zones. neto loss-of-function phenotypes parallel the loss-of-function defects described for iGluRs. The defects in neto mutants are effectively rescued by muscle-specific expression of neto transgenes. Neto clustering at the Drosophila NMJ coincides with and is dependent on iGluRs. Our studies reveal that Drosophila Neto is a novel, essential component of the iGluR complexes and is required for iGluR clustering, organization of PSDs, and synapse functionality.

Links

PubMed PMC3347794 Online version:10.1101/gad.185165.111

Keywords

Animals; Drosophila Proteins/genetics; Drosophila Proteins/metabolism; Drosophila melanogaster/metabolism; Membrane Proteins/genetics; Membrane Proteins/metabolism; Muscle, Striated/metabolism; Neuromuscular Junction/metabolism; Post-Synaptic Density/metabolism; Receptors, Glutamate/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:G4LU05

located_in

GO:0031594: neuromuscular junction

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:G4LU05

located_in

GO:0097482: muscle cell postsynaptic specialization

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:G4LU05

involved_in

GO:0007528: neuromuscular junction development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:G4LU05

involved_in

GO:0007274: neuromuscular synaptic transmission

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:G4LU05

part_of

GO:0008328: ionotropic glutamate receptor complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:G4LU05

involved_in

GO:0060361: flight

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:G4LU05

involved_in

GO:0043113: receptor clustering

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:G4LU05

involved_in

GO:0040011: locomotion

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:G4LU05

involved_in

GO:0097106: postsynaptic density organization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:G4LU05

involved_in

GO:0035187: hatching behavior

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9VYC7

located_in

GO:0031594: neuromuscular junction

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:Q9VYC7

located_in

GO:0097482: muscle cell postsynaptic specialization

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:Q9VYC7

involved_in

GO:0007528: neuromuscular junction development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9VYC7

involved_in

GO:0007274: neuromuscular synaptic transmission

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9VYC7

part_of

GO:0008328: ionotropic glutamate receptor complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:Q9VYC7

involved_in

GO:0060361: flight

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9VYC7

involved_in

GO:0043113: receptor clustering

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9VYC7

involved_in

GO:0040011: locomotion

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9VYC7

involved_in

GO:0097106: postsynaptic density organization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9VYC7

involved_in

GO:0035187: hatching behavior

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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