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PMID:22493317
| Citation |
Backer, CB, Gutzman, JH, Pearson, CG and Cheeseman, IM (2012) CSAP localizes to polyglutamylated microtubules and promotes proper cilia function and zebrafish development. Mol. Biol. Cell 23:2122-30 |
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| Abstract |
The diverse populations of microtubule polymers in cells are functionally distinguished by different posttranslational modifications, including polyglutamylation. Polyglutamylation is enriched on subsets of microtubules including those found in the centrioles, mitotic spindle, and cilia. However, whether this modification alters intrinsic microtubule dynamics or affects extrinsic associations with specific interacting partners remains to be determined. Here we identify the microtubule-binding protein centriole and spindle-associated protein (CSAP), which colocalizes with polyglutamylated tubulin to centrioles, spindle microtubules, and cilia in human tissue culture cells. Reducing tubulin polyglutamylation prevents CSAP localization to both spindle and cilia microtubules. In zebrafish, CSAP is required for normal brain development and proper left-right asymmetry, defects that are qualitatively similar to those reported previously for depletion of polyglutamylation-conjugating enzymes. We also find that CSAP is required for proper cilia beating. Our work supports a model in which polyglutamylation can target selected microtubule-associated proteins, such as CSAP, to microtubule subpopulations, providing specific functional capabilities to these populations. |
| Links |
PubMed PMC3364176 Online version:10.1091/mbc.E11-11-0931 |
| Keywords |
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| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
HUMAN:CCSAP |
located_in |
GO:0005819: spindle |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
HUMAN:CCSAP |
located_in |
GO:0005929: cilium |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
HUMAN:CCSAP |
located_in |
GO:0005814: centriole |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
HUMAN:CCSAP |
located_in |
GO:0036064: ciliary basal body |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
HUMAN:CCSAP |
located_in |
GO:0030424: axon |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
HUMAN:CCSAP |
located_in |
GO:0005930: axoneme |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
HUMAN:CCSAP |
located_in |
GO:0035869: ciliary transition zone |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
DANRE:CCSAP |
involved_in |
GO:0048666: neuron development |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
DANRE:CCSAP |
involved_in |
GO:0007507: heart development |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
DANRE:CCSAP |
involved_in |
GO:0007420: brain development |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
DANRE:CCSAP |
located_in |
GO:0036064: ciliary basal body |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
DANRE:CCSAP |
involved_in |
GO:0060296: regulation of cilium beat frequency involved in ciliary motility |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
DANRE:CCSAP |
involved_in |
GO:0045995: regulation of embryonic development |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
DANRE:CCSAP |
involved_in |
GO:0060548: negative regulation of cell death |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
DANRE:CCSAP |
involved_in |
GO:0045930: negative regulation of mitotic cell cycle |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
DANRE:CCSAP |
involved_in |
GO:0061371: determination of heart left/right asymmetry |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
DANRE:CCSAP |
involved_in |
GO:0007420: brain development |
ECO:0000315: mutant phenotype evidence used in manual assertion |
ZFIN:ZDB-MRPHLNO-120611-2 |
P |
Seeded From UniProt |
complete | |
|
DANRE:CCSAP |
involved_in |
GO:0003341: cilium movement |
ECO:0000315: mutant phenotype evidence used in manual assertion |
ZFIN:ZDB-MRPHLNO-120611-2 |
P |
Seeded From UniProt |
complete | |
|
DANRE:CCSAP |
involved_in |
GO:0061371: determination of heart left/right asymmetry |
ECO:0000315: mutant phenotype evidence used in manual assertion |
ZFIN:ZDB-MRPHLNO-120611-2 |
P |
Seeded From UniProt |
complete | |
See also
References
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