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PMID:22461611

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Citation

Liu, F, Bakht, S and Dean, C (2012) Cotranscriptional role for Arabidopsis DICER-LIKE 4 in transcription termination. Science 335:1621-3

Abstract

Transcription termination is emerging as an important component of gene regulation necessary to partition the genome and minimize transcriptional interference. We have discovered a role for the Arabidopsis RNA silencing enzyme DICER-LIKE 4 (DCL4) in transcription termination of an endogenous Arabidopsis gene, FCA. DCL4 directly associates with FCA chromatin in the 3' region and promotes cleavage of the nascent transcript in a domain downstream of the canonical polyA site. In a dcl4 mutant, the resulting transcriptional read-through triggers an RNA interference-mediated gene silencing of a transgene containing the same 3' region. We conclude that DCL4 promotes transcription termination of the Arabidopsis FCA gene, reducing the amount of aberrant RNA produced from the locus.

Links

PubMed Online version:10.1126/science.1214402

Keywords

Arabidopsis/genetics; Arabidopsis/metabolism; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Base Sequence; Chromatin/genetics; Chromatin/metabolism; Chromatin Immunoprecipitation; Gene Expression Regulation, Plant; MADS Domain Proteins/genetics; MADS Domain Proteins/metabolism; Molecular Sequence Data; Mutation; Polyadenylation; Protein Structure, Tertiary; RNA Interference; RNA, Messenger/genetics; RNA, Messenger/metabolism; RNA, Plant/genetics; RNA, Plant/metabolism; RNA-Binding Proteins/genetics; RNA-Binding Proteins/metabolism; Ribonuclease III/chemistry; Ribonuclease III/genetics; Ribonuclease III/metabolism; Transcription, Genetic; Transgenes

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:DCL4

involved_in

GO:0006353: DNA-templated transcription, termination

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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