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PMID:22438917

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Citation

Chen, SJ, Wu, YH, Huang, HY and Wang, CC (2012) Saccharomyces cerevisiae possesses a stress-inducible glycyl-tRNA synthetase gene. PLoS ONE 7:e33363

Abstract

Aminoacyl-tRNA synthetases are a large family of housekeeping enzymes that are pivotal in protein translation and other vital cellular processes. Saccharomyces cerevisiae possesses two distinct nuclear glycyl-tRNA synthetase (GlyRS) genes, GRS1 and GRS2. GRS1 encodes both cytoplasmic and mitochondrial activities, while GRS2 is essentially silent and dispensable under normal conditions. We herein present evidence that expression of GRS2 was drastically induced upon heat shock, ethanol or hydrogen peroxide addition, and high pH, while expression of GRS1 was somewhat repressed under those conditions. In addition, GlyRS2 (the enzyme encoded by GRS2) had a higher protein stability and a lower K(M) value for yeast tRNA(Gly) under heat shock conditions than under normal conditions. Moreover, GRS2 rescued the growth defect of a GRS1 knockout strain when highly expressed by a strong promoter at 37 °C, but not at the optimal temperature of 30 °C. These results suggest that GRS2 is actually an inducible gene that may function to rescue the activity of GRS1 under stress conditions.

Links

PubMed PMC3306390 Online version:10.1371/journal.pone.0033363

Keywords

Amino Acid Sequence; Enzyme Induction; Enzyme Stability; Gene Knockout Techniques; Genes, Fungal; Glycine-tRNA Ligase/biosynthesis; Glycine-tRNA Ligase/chemistry; Glycine-tRNA Ligase/genetics; Kinetics; Molecular Sequence Data; Saccharomyces cerevisiae/enzymology; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae Proteins/biosynthesis; Saccharomyces cerevisiae Proteins/chemistry; Saccharomyces cerevisiae Proteins/genetics; Sequence Homology, Amino Acid; Stress, Physiological; Yeasts/enzymology; Yeasts/genetics

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:SYG2

involved_in

GO:0006426: glycyl-tRNA aminoacylation

ECO:0000316: genetic interaction evidence used in manual assertion

SGD:S000000325

P

Seeded From UniProt

complete

YEAST:SYG2

enables

GO:0004820: glycine-tRNA ligase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

YEAST:SYG2

enables

GO:0004820: glycine-tRNA ligase activity

ECO:0000316: genetic interaction evidence used in manual assertion

SGD:S000000325

F

Seeded From UniProt

complete


See also

References

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