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PMID:22358839

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Citation

Jin, L, Pahuja, KB, Wickliffe, KE, Gorur, A, Baumgärtel, C, Schekman, R and Rape, M (2012) Ubiquitin-dependent regulation of COPII coat size and function. Nature 482:495-500

Abstract

Packaging of proteins from the endoplasmic reticulum into COPII vesicles is essential for secretion. In cells, most COPII vesicles are approximately 60-80 nm in diameter, yet some must increase their size to accommodate 300-400 nm procollagen fibres or chylomicrons. Impaired COPII function results in collagen deposition defects, cranio-lenticulo-sutural dysplasia, or chylomicron retention disease, but mechanisms to enlarge COPII coats have remained elusive. Here, we identified the ubiquitin ligase CUL3-KLHL12 as a regulator of COPII coat formation. CUL3-KLHL12 catalyses the monoubiquitylation of the COPII-component SEC31 and drives the assembly of large COPII coats. As a result, ubiquitylation by CUL3-KLHL12 is essential for collagen export, yet less important for the transport of small cargo. We conclude that monoubiquitylation controls the size and function of a vesicle coat.

Links

PubMed PMC3292188 Online version:10.1038/nature10822

Keywords

Animals; COP-Coated Vesicles/chemistry; COP-Coated Vesicles/metabolism; Cell Line; Cell Shape; Collagen/metabolism; Cullin Proteins/genetics; Cullin Proteins/metabolism; Embryonic Stem Cells/cytology; Embryonic Stem Cells/metabolism; HeLa Cells; Humans; Mice; Microfilament Proteins/genetics; Microfilament Proteins/metabolism; Protein Transport; Ubiquitin/metabolism; Ubiquitination; Vesicular Transport Proteins/genetics; Vesicular Transport Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:KLH12

involved_in

GO:0048208: COPII vesicle coating

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:KLH12

part_of

GO:0031463: Cul3-RING ubiquitin ligase complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:KLH12

part_of

GO:0030134: COPII-coated ER to Golgi transport vesicle

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:KLH12

involved_in

GO:0006888: endoplasmic reticulum to Golgi vesicle-mediated transport

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:KLH12

involved_in

GO:0006513: protein monoubiquitination

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:CUL3

involved_in

GO:0006513: protein monoubiquitination

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:CUL3

involved_in

GO:0006888: endoplasmic reticulum to Golgi vesicle-mediated transport

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:CUL3

part_of

GO:0031463: Cul3-RING ubiquitin ligase complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:CUL3

involved_in

GO:0048208: COPII vesicle coating

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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