GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:22357932

From GONUTS
Jump to: navigation, search
Citation

Alten, L, Schuster-Gossler, K, Beckers, A, Groos, S, Ulmer, B, Hegermann, J, Ochs, M and Gossler, A (2012) Differential regulation of node formation, nodal ciliogenesis and cilia positioning by Noto and Foxj1. Development 139:1276-84

Abstract

The mouse transcription factor Noto is expressed in the node and controls node morphogenesis, formation of nodal cilia and left-right asymmetry. Noto acts upstream of Foxj1, which regulates ciliogenesis in other mouse tissues. However, the significance of Foxj1 for the formation of cilia in the mouse node is unclear; in non-amniote species Foxj1 is required for ciliogenesis in the structures equivalent to the node. Here, we analyzed nodes, nodal cilia and nodal flow in mouse embryos in which we replaced the Noto-coding sequence with that of Foxj1, or in embryos that were deficient for Foxj1. We show that Foxj1 expressed from the Noto locus is functional and restores the formation of structurally normal motile cilia in the absence of Noto. However, Foxj1 is not sufficient for the correct positioning of cilia on the cell surface within the plane of the nodal epithelium, and cannot restore normal node morphology. We also show that Foxj1 is essential for ciliogenesis upstream of Rfx3 in the node. Thus, the function of Foxj1 in vertebrate organs of asymmetry is conserved, and Noto regulates node morphogenesis and the posterior localization of cilia on node cells independently of Foxj1.

Links

PubMed Online version:10.1242/dev.072728

Keywords

Alleles; Animals; Cilia/physiology; Epithelium/metabolism; Forkhead Transcription Factors/physiology; Gene Expression Regulation, Developmental; Genotype; Homeodomain Proteins/physiology; Mice; Mice, Transgenic; Microscopy, Video/methods; Models, Biological; Models, Genetic; Morphogenesis/genetics; Primitive Streak/physiology; Transcription Factors/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:NOTO

acts_upstream_of_or_within

GO:0007368: determination of left/right symmetry

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:3053092

P

Seeded From UniProt

complete

MOUSE:NOTO

acts_upstream_of_or_within

GO:0044458: motile cilium assembly

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:1347474

P

  • occurs_in:(EMAPA:16075)

Seeded From UniProt

complete

MOUSE:FOXJ1

acts_upstream_of_or_within

GO:0044458: motile cilium assembly

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:3053002

P

  • occurs_in:(EMAPA:16075)

Seeded From UniProt

complete

MOUSE:FOXJ1

acts_upstream_of_or_within

GO:0044458: motile cilium assembly

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:3053002

P

occurs_in:(EMAPA:16075)

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.