GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:22355605

From GONUTS
Jump to: navigation, search
Citation

Pulzova, L, Kovac, A, Mucha, R, Mlynarcik, P, Bencurova, E, Madar, M, Novak, M and Bhide, M (2011) OspA-CD40 dyad: ligand-receptor interaction in the translocation of neuroinvasive Borrelia across the blood-brain barrier. Sci Rep 1:86

Abstract

Lyme borreliosis is the most widespread vector-borne disease in temperate zones of Europe and North America. Although the infection is treatable, the symptoms are often overlooked resulting in infection of the neuronal system. In this work we uncover the underlying molecular mechanism of borrelial translocation across the blood-brain barrier (BBB). We demonstrate that neuroinvasive strain of Borrelia readily crosses monolayer of brain-microvascular endothelial cells (BMECs) in vitro and BBB in vivo. Using protein-protein interaction assays we found that CD40 of BMECs and OspA of Borrelia are the primary molecules in transient tethering of Borrelia to endothelium. OspA of neuroinvasive Borrelia, but not of non-neuroinvasive strain, binds CD40. Furthermore, only the neuroinvasive Borrelia and its recombinant OspA activated CD40-dependent pathway in BMECs and induced expression of integrins essential for stationary adhesion. Demonstration of the CD40-ligand interactions may provide a new possible perspective on molecular mechanisms of borrelial BBB translocation process.

Links

PubMed PMC3216572 Online version:10.1038/srep00086

Keywords


Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

RAT:Q4QQW2

involved_in

GO:0009617: response to bacterium

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

RAT:Q4QQW2

enables

GO:0003823: antigen binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

BORBU:OSPA7

involved_in

GO:0007155: cell adhesion

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

BORBG:OSPA7

GO:0007155: cell adhesion

ECO:0000314:

P

figure 2 demonstrated the adhesion of OspA proteins to CD40 molecules in brain-microvascular endothelia cells, showing that Correlial adhesion to the endothelial cells

complete
CACAO 3976

BORBG:OSPA7

involved_in

GO:0007155: cell adhesion

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.