PMID:22325148

Citation	Tavares, L, Dimitrova, E, Oxley, D, Webster, J, Poot, R, Demmers, J, Bezstarosti, K, Taylor, S, Ura, H, Koide, H, Wutz, A, Vidal, M, Elderkin, S and Brockdorff, N (2012) RYBP-PRC1 complexes mediate H2A ubiquitylation at polycomb target sites independently of PRC2 and H3K27me3. Cell 148:664-78
Abstract	Polycomb-repressive complex 1 (PRC1) has a central role in the regulation of heritable gene silencing during differentiation and development. PRC1 recruitment is generally attributed to interaction of the chromodomain of the core protein Polycomb with trimethyl histone H3K27 (H3K27me3), catalyzed by a second complex, PRC2. Unexpectedly we find that RING1B, the catalytic subunit of PRC1, and associated monoubiquitylation of histone H2A are targeted to closely overlapping sites in wild-type and PRC2-deficient mouse embryonic stem cells (mESCs), demonstrating an H3K27me3-independent pathway for recruitment of PRC1 activity. We show that this pathway is mediated by RYBP-PRC1, a complex comprising catalytic subunits of PRC1 and the protein RYBP. RYBP-PRC1 is recruited to target loci in mESCs and is also involved in Xist RNA-mediated silencing, the latter suggesting a wider role in Polycomb silencing. We discuss the implications of these findings for understanding recruitment and function of Polycomb repressors.
Links	PubMed PMC3281992 Online version:10.1016/j.cell.2011.12.029
Keywords	Animals; Cell Line; Embryonic Stem Cells/metabolism; Fibroblasts/metabolism; Histones/metabolism; Mice; Repressor Proteins/genetics; Repressor Proteins/metabolism; Ubiquitin-Protein Ligases/metabolism; Ubiquitination
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
MOUSE:RING1	enables	GO:0005515: protein binding	ECO:0000353: physical interaction evidence used in manual assertion	UniProtKB:Q8CCI5	F		Seeded From UniProt	complete
MOUSE:PCGF2	enables	GO:0005515: protein binding	ECO:0000353: physical interaction evidence used in manual assertion	UniProtKB:Q64028	F		Seeded From UniProt	complete
MOUSE:PCGF2	enables	GO:0005515: protein binding	ECO:0000353: physical interaction evidence used in manual assertion	UniProtKB:Q9CQJ4	F	occurs_in:(GO:0005634)|occurs_in:(GO:0016607)	Seeded From UniProt	complete
MOUSE:PCGF2	enables	GO:0005515: protein binding	ECO:0000353: physical interaction evidence used in manual assertion	UniProtKB:Q9QWH1	F		Seeded From UniProt	complete
MOUSE:PHC1	enables	GO:0005515: protein binding	ECO:0000353: physical interaction evidence used in manual assertion	UniProtKB:P23798	F		Seeded From UniProt	complete
MOUSE:PHC1	enables	GO:0005515: protein binding	ECO:0000353: physical interaction evidence used in manual assertion	UniProtKB:Q9CQJ4	F	occurs_in:(GO:0005634)	Seeded From UniProt	complete
MOUSE:RYBP	enables	GO:0005515: protein binding	ECO:0000353: physical interaction evidence used in manual assertion	UniProtKB:O35730	F		Seeded From UniProt	complete
MOUSE:RYBP	enables	GO:0005515: protein binding	ECO:0000353: physical interaction evidence used in manual assertion	UniProtKB:Q9CQJ4	F	occurs_in:(GO:0005634)	Seeded From UniProt	complete
MOUSE:RING2	enables	GO:0005515: protein binding	ECO:0000353: physical interaction evidence used in manual assertion	UniProtKB:P23798	F	occurs_in:(GO:0005634)|occurs_in:(GO:0016607)	Seeded From UniProt	complete
MOUSE:RING2	enables	GO:0005515: protein binding	ECO:0000353: physical interaction evidence used in manual assertion	UniProtKB:Q64028	F	occurs_in:(GO:0005634)	Seeded From UniProt	complete
MOUSE:RING2	enables	GO:0005515: protein binding	ECO:0000353: physical interaction evidence used in manual assertion	UniProtKB:Q8CCI5	F	occurs_in:(GO:0005634)	Seeded From UniProt	complete
MOUSE:PHC2	enables	GO:0005515: protein binding	ECO:0000353: physical interaction evidence used in manual assertion	UniProtKB:P23798	F		Seeded From UniProt	complete

References

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