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PMID:22325148

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Citation

Tavares, L, Dimitrova, E, Oxley, D, Webster, J, Poot, R, Demmers, J, Bezstarosti, K, Taylor, S, Ura, H, Koide, H, Wutz, A, Vidal, M, Elderkin, S and Brockdorff, N (2012) RYBP-PRC1 complexes mediate H2A ubiquitylation at polycomb target sites independently of PRC2 and H3K27me3. Cell 148:664-78

Abstract

Polycomb-repressive complex 1 (PRC1) has a central role in the regulation of heritable gene silencing during differentiation and development. PRC1 recruitment is generally attributed to interaction of the chromodomain of the core protein Polycomb with trimethyl histone H3K27 (H3K27me3), catalyzed by a second complex, PRC2. Unexpectedly we find that RING1B, the catalytic subunit of PRC1, and associated monoubiquitylation of histone H2A are targeted to closely overlapping sites in wild-type and PRC2-deficient mouse embryonic stem cells (mESCs), demonstrating an H3K27me3-independent pathway for recruitment of PRC1 activity. We show that this pathway is mediated by RYBP-PRC1, a complex comprising catalytic subunits of PRC1 and the protein RYBP. RYBP-PRC1 is recruited to target loci in mESCs and is also involved in Xist RNA-mediated silencing, the latter suggesting a wider role in Polycomb silencing. We discuss the implications of these findings for understanding recruitment and function of Polycomb repressors.

Links

PubMed PMC3281992 Online version:10.1016/j.cell.2011.12.029

Keywords

Animals; Cell Line; Embryonic Stem Cells/metabolism; Fibroblasts/metabolism; Histones/metabolism; Mice; Repressor Proteins/genetics; Repressor Proteins/metabolism; Ubiquitin-Protein Ligases/metabolism; Ubiquitination

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:RING1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q8CCI5

F

Seeded From UniProt

complete

MOUSE:PCGF2

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q64028

F

Seeded From UniProt

complete

MOUSE:PCGF2

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q9CQJ4

F

Seeded From UniProt

complete

MOUSE:PCGF2

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q9QWH1

F

Seeded From UniProt

complete

MOUSE:PHC1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P23798

F

Seeded From UniProt

complete

MOUSE:PHC1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q9CQJ4

F

Seeded From UniProt

complete

MOUSE:RYBP

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:O35730

F

Seeded From UniProt

complete

MOUSE:RYBP

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q9CQJ4

F

Seeded From UniProt

complete

MOUSE:RING2

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P23798

F

Seeded From UniProt

complete

MOUSE:RING2

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q64028

F

Seeded From UniProt

complete

MOUSE:RING2

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q8CCI5

F

Seeded From UniProt

complete

MOUSE:PHC2

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P23798

F

Seeded From UniProt

complete


See also

References

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