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PMID:22319053

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Citation

Sosso, D, Mbelo, S, Vernoud, V, Gendrot, G, Dedieu, A, Chambrier, P, Dauzat, M, Heurtevin, L, Guyon, V, Takenaka, M and Rogowsky, PM (2012) PPR2263, a DYW-Subgroup Pentatricopeptide repeat protein, is required for mitochondrial nad5 and cob transcript editing, mitochondrion biogenesis, and maize growth. Plant Cell 24:676-91

Abstract

RNA editing plays an important role in organelle gene expression in various organisms, including flowering plants, changing the nucleotide information at precise sites. Here, we present evidence that the maize (Zea mays) nuclear gene Pentatricopeptide repeat 2263 (PPR2263) encoding a DYW domain-containing PPR protein is required for RNA editing in the mitochondrial NADH dehydrogenase5 (nad5) and cytochrome b (cob) transcripts at the nad5-1550 and cob-908 sites, respectively. Its putative ortholog, MITOCHONDRIAL EDITING FACTOR29, fulfills the same role in Arabidopsis thaliana. Both the maize and the Arabidopsis proteins show preferential localization to mitochondria but are also detected in chloroplasts. In maize, the corresponding ppr2263 mutation causes growth defects in kernels and seedlings. Embryo and endosperm growth are reduced, leading to the production of small but viable kernels. Mutant plants have narrower and shorter leaves, exhibit a strong delay in flowering time, and generally do not reach sexual maturity. Whereas mutant chloroplasts do not have major defects, mutant mitochondria lack complex III and are characterized by a compromised ultrastructure, increased transcript levels, and the induction of alternative oxidase. The results suggest that mitochondrial RNA editing at the cob-908 site is necessary for mitochondrion biogenesis, cell division, and plant growth in maize.

Links

PubMed PMC3315240 Online version:10.1105/tpc.111.091074

Keywords

Amino Acid Sequence; Arabidopsis/genetics; Arabidopsis/growth & development; Chloroplasts/enzymology; Cytochromes b/genetics; Gene Expression Regulation, Plant; Microscopy, Electron, Transmission; Mitochondria/enzymology; Mitochondria/ultrastructure; Mitochondrial Proteins/genetics; Mitochondrial Proteins/metabolism; Molecular Sequence Data; Mutagenesis, Insertional; NADH Dehydrogenase/genetics; Oxidoreductases/metabolism; Phenotype; Plant Proteins/genetics; Plant Proteins/metabolism; Plants, Genetically Modified/genetics; Plants, Genetically Modified/metabolism; RNA Editing; RNA, Plant/genetics; Seeds/growth & development; Zea mays/genetics; Zea mays/growth & development; Zea mays/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:PP341

located_in

GO:0009507: chloroplast

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:PP341

located_in

GO:0005739: mitochondrion

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:PP341

acts_upstream_of_or_within

GO:0080156: mitochondrial mRNA modification

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:PP341

acts_upstream_of_or_within

GO:0016554: cytidine to uridine editing

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:PP341

acts_upstream_of_or_within

GO:0000963: mitochondrial RNA processing

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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