GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:22312005

From GONUTS
Jump to: navigation, search
Citation

Ladwig, F, Stahl, M, Ludewig, U, Hirner, AA, Hammes, UZ, Stadler, R, Harter, K and Koch, W (2012) Siliques are Red1 from Arabidopsis acts as a bidirectional amino acid transporter that is crucial for the amino acid homeostasis of siliques. Plant Physiol. 158:1643-55

Abstract

Many membrane proteins are involved in the transport of nutrients in plants. While the import of amino acids into plant cells is, in principle, well understood, their export has been insufficiently described. Here, we present the identification and characterization of the membrane protein Siliques Are Red1 (SIAR1) from Arabidopsis (Arabidopsis thaliana) that is able to translocate amino acids bidirectionally into as well as out of the cell. Analyses in yeast and oocytes suggest a SIAR1-mediated export of amino acids. In Arabidopsis, SIAR1 localizes to the plasma membrane and is expressed in the vascular tissue, in the pericycle, in stamen, and in the chalazal seed coat of ovules and developing seeds. Mutant alleles of SIAR1 accumulate anthocyanins as a symptom of reduced amino acid content in the early stages of silique development. Our data demonstrate that the SIAR1-mediated export of amino acids plays an important role in organic nitrogen allocation and particularly in amino acid homeostasis in developing siliques.

Links

PubMed PMC3320175 Online version:10.1104/pp.111.192583

Keywords

Amino Acid Transport Systems/genetics; Amino Acid Transport Systems/metabolism; Amino Acids/genetics; Amino Acids/metabolism; Animals; Arabidopsis/metabolism; Arabidopsis Proteins/metabolism; Biological Transport; Cell Membrane/metabolism; Genetic Complementation Test; Green Fluorescent Proteins/metabolism; Homeostasis; Multigene Family; Mutation/genetics; Oocytes/metabolism; Organ Specificity; Oryza sativa; Phenotype; Phylogeny; Plant Vascular Bundle/metabolism; Recombinant Fusion Proteins/metabolism; Saccharomyces cerevisiae/metabolism; Seeds/metabolism; Subcellular Fractions/metabolism; Xenopus laevis

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:WTR8

enables

GO:0034639: L-amino acid efflux transmembrane transporter activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:WTR8

acts_upstream_of_or_within

GO:0032973: amino acid export across plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:WTR8

acts_upstream_of_or_within

GO:0006865: amino acid transport

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:WTR8

located_in

GO:0005886: plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:WTR8

enables

GO:0015171: amino acid transmembrane transporter activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:WTR8

acts_upstream_of_or_within

GO:0080144: amino acid homeostasis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:WTR8

acts_upstream_of_or_within

GO:0043090: amino acid import

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.