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PMID:22307851

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Citation

Moreau, M, Azzopardi, M, Clément, G, Dobrenel, T, Marchive, C, Renne, C, Martin-Magniette, ML, Taconnat, L, Renou, JP, Robaglia, C and Meyer, C (2012) Mutations in the Arabidopsis homolog of LST8/GβL, a partner of the target of Rapamycin kinase, impair plant growth, flowering, and metabolic adaptation to long days. Plant Cell 24:463-81

Abstract

The conserved Target of Rapamycin (TOR) kinase forms high molecular mass complexes and is a major regulator of cellular adaptations to environmental cues. The Lethal with Sec Thirteen 8/G protein β subunit-like (LST8/GβL) protein is a member of the TOR complexes, and two putative LST8 genes are present in Arabidopsis thaliana, of which only one (LST8-1) is significantly expressed. The Arabidopsis LST8-1 protein is able to complement yeast lst8 mutations and interacts with the TOR kinase. Mutations in the LST8-1 gene resulted in reduced vegetative growth and apical dominance with abnormal development of flowers. Mutant plants were also highly sensitive to long days and accumulated, like TOR RNA interference lines, higher amounts of starch and amino acids, including proline and glutamine, while showing reduced concentrations of inositol and raffinose. Accordingly, transcriptomic and enzymatic analyses revealed a higher expression of genes involved in nitrate assimilation when lst8-1 mutants were shifted to long days. The transcriptome of lst8-1 mutants in long days was found to share similarities with that of a myo-inositol 1 phosphate synthase mutant that is also sensitive to the extension of the light period. It thus appears that the LST8-1 protein has an important role in regulating amino acid accumulation and the synthesis of myo-inositol and raffinose during plant adaptation to long days.

Links

PubMed PMC3315227 Online version:10.1105/tpc.111.091306

Keywords

Acclimatization/genetics; Amino Acids/metabolism; Arabidopsis/genetics; Arabidopsis/growth & development; Arabidopsis/metabolism; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Flowers/growth & development; Gene Expression Regulation, Plant; Metabolome; Mutation; Phosphatidylinositol 3-Kinases/metabolism; Photoperiod; Plants, Genetically Modified/genetics; Plants, Genetically Modified/growth & development; Plants, Genetically Modified/metabolism; RNA Interference; Transcriptome

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:TOR

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q9LV27

F

Seeded From UniProt

complete

ARATH:LST81

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q9FR53

F

Seeded From UniProt

complete

ARATH:LST81

located_in

GO:0016020: membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:LST81

located_in

GO:0005768: endosome

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:LST81

acts_upstream_of_or_within

GO:0048571: long-day photoperiodism

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:LST81

acts_upstream_of_or_within

GO:1900091: regulation of raffinose biosynthetic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:LST81

acts_upstream_of_or_within

GO:1900088: regulation of inositol biosynthetic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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