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PMID:22285184

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Citation

Jung, I, Sohn, DH, Choi, J, Kim, JM, Jeon, S, Seol, JH and Seong, RH (2012) SRG3/mBAF155 stabilizes the SWI/SNF-like BAF complex by blocking CHFR mediated ubiquitination and degradation of its major components. Biochem. Biophys. Res. Commun. 418:512-7

Abstract

The murine SWI/SNF-like BAF complex is an ATP-dependent chromatin remodeling complex that functions as a transcriptional regulator in cell proliferation, differentiation and development. The SWI/SNF-like BAF complex consists of several components including core subunits such as BRG1, BAF155/SRG3, BAF47/SNF5/INI1, and BAF170. We have previously shown that the interaction between SRG3/mBAF155 and other components of the complex stabilizes them by attenuating their proteasomal degradation. However, it has not been known how the major components of the SWI/SNF-like BAF complex such as BRG1, SNF5, and BAF60a are targeted for the ubiquitination and degradation, and how SRG3/mBAF155 protects them from the degradation process. Here we report that CHFR interacts with BRG1, SNF5, and BAF60a of the SWI/SNF-like BAF complex and ubiquitinates them to target for degradation through a proteasome-mediated pathway, and that SRG3/mBAF155 stabilizes these components by blocking their interaction with CHFR.

Links

PubMed Online version:10.1016/j.bbrc.2012.01.057

Keywords

Animals; COS Cells; Cercopithecus aethiops; Chromosomal Proteins, Non-Histone/metabolism; DNA Helicases/metabolism; Enzyme Stability; Humans; Mice; Nuclear Proteins/metabolism; Proteolysis; Transcription Factors/genetics; Transcription Factors/metabolism; Tumor Suppressor Proteins/metabolism; Ubiquitin-Protein Ligases/metabolism; Ubiquitination

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:SMRC1

acts_upstream_of_or_within

GO:0032435: negative regulation of proteasomal ubiquitin-dependent protein catabolic process

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:SMRC1

acts_upstream_of_or_within

GO:0032435: negative regulation of proteasomal ubiquitin-dependent protein catabolic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:SMCA4

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:Q810L3

F

Seeded From UniProt

complete

MOUSE:SMRD1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:Q810L3

F

Seeded From UniProt

complete

MOUSE:CHFR

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:Q3TKT4
PR:Q61466
PR:Q9Z0H3

F

Seeded From UniProt

complete

MOUSE:SNF5

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:Q810L3

F

Seeded From UniProt

complete

MOUSE:CHFR

acts_upstream_of_or_within

GO:0031398: positive regulation of protein ubiquitination

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:CHFR

acts_upstream_of_or_within

GO:0031648: protein destabilization

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:CHFR

acts_upstream_of_or_within

GO:0032436: positive regulation of proteasomal ubiquitin-dependent protein catabolic process

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

Notes

See also

References

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