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PMID:22253597

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Citation

Cottier, F, Raymond, M, Kurzai, O, Bolstad, M, Leewattanapasuk, W, Jiménez-López, C, Lorenz, MC, Sanglard, D, Váchová, L, Pavelka, N, Palková, Z and Mühlschlegel, FA (2012) The bZIP transcription factor Rca1p is a central regulator of a novel CO₂ sensing pathway in yeast. PLoS Pathog. 8:e1002485

Abstract

Like many organisms the fungal pathogen Candida albicans senses changes in the environmental CO(2) concentration. This response involves two major proteins: adenylyl cyclase and carbonic anhydrase (CA). Here, we demonstrate that CA expression is tightly controlled by the availability of CO(2) and identify the bZIP transcription factor Rca1p as the first CO(2) regulator of CA expression in yeast. We show that Rca1p upregulates CA expression during contact with mammalian phagocytes and demonstrate that serine 124 is critical for Rca1p signaling, which occurs independently of adenylyl cyclase. ChIP-chip analysis and the identification of Rca1p orthologs in the model yeast Saccharomyces cerevisiae (Cst6p) point to the broad significance of this novel pathway in fungi. By using advanced microscopy we visualize for the first time the impact of CO(2) build-up on gene expression in entire fungal populations with an exceptional level of detail. Our results present the bZIP protein Rca1p as the first fungal regulator of carbonic anhydrase, and reveal the existence of an adenylyl cyclase independent CO(2) sensing pathway in yeast. Rca1p appears to regulate cellular metabolism in response to CO(2) availability in environments as diverse as the phagosome, yeast communities or liquid culture.

Links

PubMed PMC3257301 Online version:10.1371/journal.ppat.1002485

Keywords

Adenosine Triphosphatases/genetics; Adenosine Triphosphatases/metabolism; Adenosine Triphosphatases/physiology; Basic-Leucine Zipper Transcription Factors/genetics; Basic-Leucine Zipper Transcription Factors/metabolism; Basic-Leucine Zipper Transcription Factors/physiology; Biota; Carbon Dioxide/metabolism; Chromatin Immunoprecipitation; Environment; Gene Expression Profiling; Gene Expression Regulation, Fungal; Metalloendopeptidases/genetics; Metalloendopeptidases/metabolism; Metalloendopeptidases/physiology; Microbiological Techniques; Mitochondrial Proteins/genetics; Mitochondrial Proteins/metabolism; Mitochondrial Proteins/physiology; Models, Biological; Oligonucleotide Array Sequence Analysis; Organisms, Genetically Modified; Phagosomes/genetics; Phagosomes/metabolism; Quorum Sensing/genetics; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae/physiology; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; Saccharomyces cerevisiae Proteins/physiology; Signal Transduction/genetics; Signal Transduction/physiology; Yeasts/genetics; Yeasts/metabolism; Yeasts/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:ACA2

involved_in

GO:0071244: cellular response to carbon dioxide

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:CAN

involved_in

GO:0071244: cellular response to carbon dioxide

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CANAL:Q5AB69

located_in

GO:0000785: chromatin

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CANAL:Q5AB69

acts_upstream_of_or_within

GO:0031505: fungal-type cell wall organization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CANAL:Q5AB69

acts_upstream_of_or_within

GO:0030447: filamentous growth

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CANAL:Q5AB69

acts_upstream_of_or_within

GO:0071244: cellular response to carbon dioxide

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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