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PMID:22245677

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Citation

Chen, G, Greer, MS, Lager, I, Yilmaz, JL, Mietkiewska, E, Carlsson, AS, Stymne, S and Weselake, RJ (2012) Identification and characterization of an LCAT-like Arabidopsis thaliana gene encoding a novel phospholipase A. FEBS Lett. 586:373-7

Abstract

A previously uncharacterized Arabidopsis lecithin:cholesterol acyltransferase (LCAT) family gene (At4g19860) was functionally expressed in yeast, where it was demonstrated to encode a novel cytosolic and calcium-independent phospholipase A with preferences for the sn-2 position. This enzyme shows optimal activity at pH 5.0, exhibits a headgroup specificity for phosphatidylcholine>phosphatidic acid>phosphatidylethanolamine>phosphatidylglycerol>phosphatidylserine and has an acyl chain specificity for oleoyl>linoleoyl>ricinoleoyl. The expression of AtLCAT-PLA inhibited yeast cell growth and fatty acid accumulation. AtLCAT-PLA transcript in Arabidopsis was detected at high levels in roots and siliques.

Links

PubMed Online version:10.1016/j.febslet.2011.12.034

Keywords

Amino Acid Sequence; Arabidopsis/enzymology; Arabidopsis/genetics; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Base Sequence; DNA, Plant/genetics; Genes, Plant; Hydrogen-Ion Concentration; Molecular Sequence Data; Phosphatidylcholine-Sterol O-Acyltransferase/genetics; Phosphatidylcholine-Sterol O-Acyltransferase/metabolism; Phosphatidylcholines/metabolism; Phospholipases A/genetics; Phospholipases A/metabolism; Phylogeny; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Saccharomyces cerevisiae/genetics; Substrate Specificity

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:LCAT4

located_in

GO:0005829: cytosol

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:LCAT4

acts_upstream_of_or_within

GO:0009395: phospholipid catabolic process

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:LCAT4

enables

GO:0004620: phospholipase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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