GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:22232253

From GONUTS
Jump to: navigation, search
Citation

Ito, D, Yoshimura, K, Ishikawa, K, Ogawa, T, Maruta, T and Shigeoka, S (2012) A comparative analysis of the molecular characteristics of the Arabidopsis CoA pyrophosphohydrolases AtNUDX11, 15, and 15a. Biosci. Biotechnol. Biochem. 76:139-47

Abstract

Coenzyme A (CoA) is an essential, ubiquitous cofactor in all biological systems, where it acts as the major acyl group carrier in various central metabolic reactions. Although much is known about CoA biosynthesis, it is unclear how the CoA pool is regulated the various cellular compartments. It has been found that the nucleoside diphosphates linked to some moiety X (Nudix) hydrolases, AtNUDX11 and 15, have pyrophosphohydrolase activity toward CoA and its derivatives. In this study we identified two alternatively spliced variants, AtNUDX15 and 15a, produced from the AtNUDX15 gene, and carried out comparative studies of the gene regulation, the kinetic parameters, and the intracellular localization of AtNUDX11, 15, and 15a. The present findings indicate that AtNUDX11 and AtNUDX15(a) function in the hydrolysis of malonyl-CoA in cytosol and succinyl-CoA in the mitochondria, respectively, suggesting their impact not only on CoA biosynthesis but also on various CoA-related pathways such as the TCA cycle.

Links

PubMed

Keywords

Alternative Splicing; Amino Acid Sequence; Animals; Arabidopsis/enzymology; Arabidopsis/genetics; Coenzyme A/metabolism; Cytosol/enzymology; Gene Expression Regulation, Plant; Kinetics; Mice; Mitochondria/enzymology; Molecular Sequence Data; Protein Transport; Pyrophosphatases/chemistry; Pyrophosphatases/genetics; Pyrophosphatases/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:NUD15

enables

GO:0010945: CoA pyrophosphatase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:NUD15

acts_upstream_of_or_within

GO:0015937: coenzyme A biosynthetic process

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:NUD15

acts_upstream_of_or_within

GO:0006104: succinyl-CoA metabolic process

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:NUD15

located_in

GO:0005739: mitochondrion

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:NUD11

enables

GO:0010945: CoA pyrophosphatase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:NUD11

located_in

GO:0005829: cytosol

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:NUD11

acts_upstream_of_or_within

GO:0015937: coenzyme A biosynthetic process

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:NUD11

acts_upstream_of_or_within

GO:2001294: malonyl-CoA catabolic process

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.