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PMID:22150160

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Citation

Seo, PJ, Park, J, Park, MJ, Kim, YS, Kim, SG, Jung, JH and Park, CM (2012) A Golgi-localized MATE transporter mediates iron homoeostasis under osmotic stress in Arabidopsis. Biochem. J. 442:551-61

Abstract

Iron is an essential micronutrient that acts as a cofactor in a wide variety of pivotal metabolic processes, such as the electron transport chain of respiration, photosynthesis and redox reactions, in plants. However, its overload exceeding the cellular capacity of iron binding and storage is potentially toxic to plant cells by causing oxidative stress and cell death. Consequently, plants have developed versatile mechanisms to maintain iron homoeostasis. Organismal iron content is tightly regulated at the steps of uptake, translocation and compartmentalization. Whereas iron uptake is fairly well understood at the cellular and organismal levels, intracellular and intercellular transport is only poorly understood. In the present study, we show that a MATE (multidrug and toxic compound extrusion) transporter, designated BCD1 (BUSH-AND-CHLOROTIC-DWARF 1), contributes to iron homoeostasis during stress responses and senescence in Arabidopsis. The BCD1 gene is induced by excessive iron, but repressed by iron deficiency. It is also induced by cellular and tissue damage occurring under osmotic stress. The activation-tagged mutant bcd1-1D exhibits leaf chlorosis, a typical symptom of iron deficiency. The chlorotic lesion of the mutant was partially recovered by iron feeding. Whereas the bcd1-1D mutant accumulated a lower amount of iron, the iron level was elevated in the knockout mutant bcd1-1. The BCD1 protein is localized to the Golgi complex. We propose that the BCD1 transporter plays a role in sustaining iron homoeostasis by reallocating excess iron released from stress-induced cellular damage.

Links

PubMed Online version:10.1042/BJ20111311

Keywords

Arabidopsis/metabolism; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Gene Expression Regulation, Plant; Golgi Apparatus/metabolism; Homeostasis; Iron/metabolism; Organic Cation Transport Proteins/genetics; Organic Cation Transport Proteins/metabolism; Osmosis/physiology; Plant Leaves/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:DTX48

involved_in

GO:0006970: response to osmotic stress

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:DTX48

involved_in

GO:0009737: response to abscisic acid

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:DTX48

located_in

GO:0005794: Golgi apparatus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:DTX48

involved_in

GO:0009646: response to absence of light

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:DTX48

involved_in

GO:0009408: response to heat

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:DTX48

enables

GO:0042910: xenobiotic transmembrane transporter activity

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:DTX48

enables

GO:0005381: iron ion transmembrane transporter activity

ECO:0000316: genetic interaction evidence used in manual assertion

UniProtKB:G0YVK8

F

Seeded From UniProt

complete

ARATH:DTX48

acts_upstream_of_or_within

GO:0006826: iron ion transport

ECO:0000316: genetic interaction evidence used in manual assertion

UniProtKB:G0YVK8

P

Seeded From UniProt

complete

ARATH:DTX48

part_of

GO:0017119: Golgi transport complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:DTX48

acts_upstream_of_or_within

GO:0055072: iron ion homeostasis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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