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PMID:22147290

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Citation

Aung, K and Hu, J (2011) The Arabidopsis tail-anchored protein PEROXISOMAL AND MITOCHONDRIAL DIVISION FACTOR1 is involved in the morphogenesis and proliferation of peroxisomes and mitochondria. Plant Cell 23:4446-61

Abstract

Peroxisomes and mitochondria are multifunctional eukaryotic organelles that are not only interconnected metabolically but also share proteins in division. Two evolutionarily conserved division factors, dynamin-related protein (DRP) and its organelle anchor FISSION1 (FIS1), mediate the fission of both peroxisomes and mitochondria. Here, we identified and characterized a plant-specific protein shared by these two types of organelles. The Arabidopsis thaliana PEROXISOMAL and MITOCHONDRIAL DIVISION FACTOR1 (PMD1) is a coiled-coil protein tethered to the membranes of peroxisomes and mitochondria by its C terminus. Null mutants of PMD1 contain enlarged peroxisomes and elongated mitochondria, and plants overexpressing PMD1 have an increased number of these organelles that are smaller in size and often aggregated. PMD1 lacks physical interaction with the known division proteins DRP3 and FIS1; it is also not required for DRP3's organelle targeting. Affinity purifications pulled down PMD1's homolog, PMD2, which exclusively targets to mitochondria and plays a specific role in mitochondrial morphogenesis. PMD1 and PMD2 can form homo- and heterocomplexes. Organelle targeting signals reside in the C termini of these proteins. Our results suggest that PMD1 facilitates peroxisomal and mitochondrial proliferation in a FIS1/DRP3-independent manner and that the homologous proteins PMD1 and PMD2 perform nonredundant functions in organelle morphogenesis.

Links

PubMed PMC3269876 Online version:10.1105/tpc.111.090142

Keywords

Agrobacterium tumefaciens/genetics; Agrobacterium tumefaciens/metabolism; Amino Acid Sequence; Arabidopsis/genetics; Arabidopsis/growth & development; Arabidopsis/physiology; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Biological Markers; Cell Proliferation; Chromatography, Affinity; Cloning, Molecular; Membrane Proteins/genetics; Membrane Proteins/metabolism; Microscopy, Electron, Transmission; Mitochondria/metabolism; Mitochondria/physiology; Mitochondrial Proteins/genetics; Mitochondrial Proteins/isolation & purification; Mitochondrial Proteins/metabolism; Molecular Sequence Data; Morphogenesis; Peroxisomes/metabolism; Peroxisomes/physiology; Plant Leaves/genetics; Plant Leaves/metabolism; Plant Leaves/ultrastructure; Plants, Genetically Modified/genetics; Plants, Genetically Modified/growth & development; Plants, Genetically Modified/physiology; Plasmids/genetics; Plasmids/metabolism; Protein Sorting Signals; Sequence Homology, Amino Acid; Tobacco/genetics; Tobacco/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:PMD1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q9SHJ6

F

Seeded From UniProt

complete

ARATH:PMD1

enables

GO:0042803: protein homodimerization activity

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q9LXR8

F

Seeded From UniProt

complete

ARATH:PMD1

located_in

GO:0031966: mitochondrial membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:PMD1

acts_upstream_of_or_within

GO:0007031: peroxisome organization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:PMD1

acts_upstream_of_or_within

GO:0007005: mitochondrion organization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:PMD1

located_in

GO:0005739: mitochondrion

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:PMD1

located_in

GO:0005778: peroxisomal membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:PMD1

located_in

GO:0005777: peroxisome

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:PMD2

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q9LXR8

F

Seeded From UniProt

complete

ARATH:PMD2

acts_upstream_of_or_within

GO:0007005: mitochondrion organization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:PMD2

located_in

GO:0005739: mitochondrion

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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