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PMID:22140429

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Citation

Zhang, H, Zhang, L, Gao, B, Fan, H, Jin, J, Botella, MA, Jiang, L and Lin, J (2011) Golgi apparatus-localized synaptotagmin 2 is required for unconventional secretion in Arabidopsis. PLoS ONE 6:e26477

Abstract

Most secretory proteins contain signal peptides that direct their sorting to the ER and secreted via the conventional ER/Golgi transport pathway, while some signal-peptide-lacking proteins have been shown to export through ER/Golgi independent secretory pathways. Hygromycin B is an aminoglycoside antibiotic produced by Streptomyces hygroscopicus that is active against both prokaryotic and eukaryotic cells. The hygromycin phosphotransferase (HYG(R)) can phosphorylate and inactivate the hygromycin B, and has been widely used as a positive selective marker in the construction of transgenic plants. However, the localization and trafficking of HYG(R) in plant cells remain unknown. Synaptotagmins (SYTs) are involved in controlling vesicle endocytosis and exocytosis as calcium sensors in animal cells, while their functions in plant cells are largely unclear.

Links

PubMed PMC3225361 Online version:10.1371/journal.pone.0026477

Keywords

Amino Acid Sequence; Arabidopsis/drug effects; Arabidopsis/genetics; Arabidopsis/metabolism; Arabidopsis/ultrastructure; Arabidopsis Proteins/chemistry; Arabidopsis Proteins/genetics; Arabidopsis Proteins/secretion; Brefeldin A/pharmacology; Cell Compartmentation/drug effects; Genes, Plant/genetics; Golgi Apparatus/drug effects; Golgi Apparatus/metabolism; Golgi Apparatus/ultrastructure; Green Fluorescent Proteins/metabolism; Hygromycin B/pharmacology; Models, Biological; Molecular Sequence Data; Mutation/genetics; Protein Transport/drug effects; Recombinant Fusion Proteins/metabolism; Secretory Pathway/drug effects; Subcellular Fractions/drug effects; Subcellular Fractions/metabolism; Synaptotagmin II/chemistry; Synaptotagmin II/genetics; Synaptotagmin II/secretion; Up-Regulation/drug effects

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:SYT2

located_in

GO:0005794: Golgi apparatus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:SYT2

acts_upstream_of_or_within

GO:0009306: protein secretion

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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