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PMID:22110953

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Citation

Gade, N, Mahapatra, RK, Sonawane, A, Singh, VK, Doreswamy, R and Saini, M (2010) Molecular Characterization of Heat Shock Protein 70-1 Gene of Goat (Capra hircus). Mol Biol Int 2010:108429

Abstract

Heat shock protein 70 (HSP 70) plays a vital role by bestowing cytoprotection against diverse kinds of stresses. The ubiquitous HSP 70 proteins are the most abundant and temperature sensitive among all the HSPs. The present paper has characterized HSP70-1 cDNA in goat (Capra hircus). Total RNA isolated from goat peripheral blood mononuclear cells was reverse transcribed to cDNA that was used for amplification of HSP 70-1 gene. PCR product (1926 bp) was cloned in pGEM-T easy vector and sequenced. Sequence analysis revealed 1926-bp-long open reading frame of HSP 70-1 gene encoding 641 amino acids in goat, as reported in cattle. At nucleotide level, goat HSP 70-1 was found to be 96-99% similar to that of sheep (partial), cattle, and buffalo whereas the similarity at amino acid level was 95-100%. Nonsynonymous substitutions exceeding synonymous substitutions indicate the evolution of this protein through positive selection among domestic animals. Goat and sheep appear to have diverged from a common ancestor in phylogenetic analysis. Predicted protein structures of goat HSP 70 protein obtained from deduced amino acid sequence indicated that the functional amino acids involved in chaperoning through ATPase hydrolytic cycle and in uncoating of clathrin coated vesicles are highly conserved.

Links

PubMed PMC3218308 Online version:10.4061/2010/108429

Keywords


Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CAPHI:C5IZH4

GO:0006950: response to stress

ECO:0000247:

UniProtKB:Q27975


P

Figure 2 shows the predicted amino acid sequence of HSP70 from goat to HSP 70 of cattle

complete
CACAO 8764

See also

References

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