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PMID:22022556

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Citation

Wakabayashi-Ito, N, Doherty, OM, Moriyama, H, Breakefield, XO, Gusella, JF, O'Donnell, JM and Ito, N (2011) Dtorsin, the Drosophila ortholog of the early-onset dystonia TOR1A (DYT1), plays a novel role in dopamine metabolism. PLoS ONE 6:e26183

Abstract

Dystonia represents the third most common movement disorder in humans. At least 15 genetic loci (DYT1-15) have been identified and some of these genes have been cloned. TOR1A (formally DYT1), the gene responsible for the most common primary hereditary dystonia, encodes torsinA, an AAA ATPase family protein. However, the function of torsinA has yet to be fully understood. Here, we have generated and characterized a complete loss-of-function mutant for dtorsin, the only Drosophila ortholog of TOR1A. Null mutation of the X-linked dtorsin was semi-lethal with most male flies dying by the pre-pupal stage and the few surviving adults being sterile and slow moving, with reduced cuticle pigmentation and thin, short bristles. Third instar male larvae exhibited locomotion defects that were rescued by feeding dopamine. Moreover, biochemical analysis revealed that the brains of third instar larvae and adults heterozygous for the loss-of-function dtorsin mutation had significantly reduced dopamine levels. The dtorsin mutant showed a very strong genetic interaction with Pu (Punch: GTP cyclohydrolase), the ortholog of the human gene underlying DYT14 dystonia. Biochemical analyses revealed a severe reduction of GTP cyclohydrolase protein and activity, suggesting that dtorsin plays a novel role in dopamine metabolism as a positive-regulator of GTP cyclohydrolase protein. This dtorsin mutant line will be valuable for understanding this relationship and potentially other novel torsin functions that could play a role in human dystonia.

Links

PubMed PMC3192163 Online version:10.1371/journal.pone.0026183

Keywords

Aging/drug effects; Aging/pathology; Animals; Brain/drug effects; Brain/metabolism; Brain/pathology; DNA, Complementary/genetics; Dopamine/metabolism; Dopamine/pharmacology; Drosophila Proteins/metabolism; Drosophila melanogaster/drug effects; Drosophila melanogaster/metabolism; Feeding Behavior/drug effects; Hemizygote; Heterozygote; Humans; Larva/drug effects; Locomotion/drug effects; Male; Molecular Chaperones/chemistry; Mutation/genetics; Neurons/drug effects; Neurons/metabolism; Phenotype; Pigmentation/drug effects; Recombination, Genetic/drug effects; Recombination, Genetic/genetics; Sequence Homology, Amino Acid

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:TORS

involved_in

GO:0048085: adult chitin-containing cuticle pigmentation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:TORS

involved_in

GO:0042053: regulation of dopamine metabolic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:TORS

involved_in

GO:0043104: positive regulation of GTP cyclohydrolase I activity

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:TORS

involved_in

GO:0040011: locomotion

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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