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PMID:21987634

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Citation

Hoppins, S, Collins, SR, Cassidy-Stone, A, Hummel, E, Devay, RM, Lackner, LL, Westermann, B, Schuldiner, M, Weissman, JS and Nunnari, J (2011) A mitochondrial-focused genetic interaction map reveals a scaffold-like complex required for inner membrane organization in mitochondria. J. Cell Biol. 195:323-40

Abstract

To broadly explore mitochondrial structure and function as well as the communication of mitochondria with other cellular pathways, we constructed a quantitative, high-density genetic interaction map (the MITO-MAP) in Saccharomyces cerevisiae. The MITO-MAP provides a comprehensive view of mitochondrial function including insights into the activity of uncharacterized mitochondrial proteins and the functional connection between mitochondria and the ER. The MITO-MAP also reveals a large inner membrane-associated complex, which we term MitOS for mitochondrial organizing structure, comprised of Fcj1/Mitofilin, a conserved inner membrane protein, and five additional components. MitOS physically and functionally interacts with both outer and inner membrane components and localizes to extended structures that wrap around the inner membrane. We show that MitOS acts in concert with ATP synthase dimers to organize the inner membrane and promote normal mitochondrial morphology. We propose that MitOS acts as a conserved mitochondrial skeletal structure that differentiates regions of the inner membrane to establish the normal internal architecture of mitochondria.

Links

PubMed PMC3198156 Online version:10.1083/jcb.201107053

Keywords

Epistasis, Genetic; Mitochondria/chemistry; Mitochondria/genetics; Mitochondrial Membranes/chemistry; Mitochondrial Proteins/chemistry; Mitochondrial Proteins/metabolism; Mitochondrial Proton-Translocating ATPases/metabolism; Multiprotein Complexes/metabolism; Yeasts/cytology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:MIC60

part_of

GO:0061617: MICOS complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:MIC60

involved_in

GO:0042407: cristae formation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:MIC12

part_of

GO:0061617: MICOS complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:MIC12

involved_in

GO:0042407: cristae formation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:MIC19

part_of

GO:0061617: MICOS complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:MIC19

involved_in

GO:0042407: cristae formation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:MIC26

part_of

GO:0061617: MICOS complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:MIC26

involved_in

GO:0042407: cristae formation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:MIC27

part_of

GO:0061617: MICOS complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:MIC27

involved_in

GO:0042407: cristae formation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:MIC10

part_of

GO:0061617: MICOS complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:MIC10

involved_in

GO:0042407: cristae formation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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