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PMID:21980417

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Citation

Wang, D, Calla, B, Vimolmangkang, S, Wu, X, Korban, SS, Huber, SC, Clough, SJ and Zhao, Y (2011) The orphan gene ybjN conveys pleiotropic effects on multicellular behavior and survival of Escherichia coli. PLoS ONE 6:e25293

Abstract

YbjN, encoding an enterobacteria-specific protein, is a multicopy suppressor of temperature sensitivity in the ts9 mutant strain of Escherichia coli. In this study, we further explored the role(s) of ybjN. First, we demonstrated that the ybjN transcript was about 10-fold lower in the ts9 strain compared to that of E. coli strain BW25113 (BW). Introduction of multiple copies of ybjN in the ts9 strain resulted in over-expression of ybjN by about 10-fold as compared to that of BW. These results suggested that temperature sensitivity of the ts9 mutant of E. coli may be related to expression levels of ybjN. Characterization of E. coli ybjN mutant revealed that ybjN mutation resulted in pleiotropic phenotypes, including increased motility, fimbriation (auto-aggregation), exopolysaccharide production, and biofilm formation. In contrast, over-expression of ybjN (in terms of multiple copies) resulted in reduced motility, fimbriation, exopolysaccharide production, biofilm formation and acid resistance. In addition, our results indicate that a ybjN-homolog gene from Erwinia amylovora, a plant enterobacterial pathogen, is functionally conserved with that of E. coli, suggesting similar evolution of the YbjN family proteins in enterobacteria. A microarray study revealed that the expression level of ybjN was inversely correlated with the expression of flagellar, fimbrial and acid resistance genes. Over-expression of ybjN significantly down-regulated genes involved in citric acid cycle, glycolysis, the glyoxylate shunt, oxidative phosphorylation, amino acid and nucleotide metabolism. Furthermore, over-expression of ybjN up-regulated toxin-antitoxin modules, the SOS response pathway, cold shock and starvation induced transporter genes. Collectively, these results suggest that YbjN may play important roles in regulating bacterial multicellular behavior, metabolism, and survival under stress conditions in E. coli. These results also suggest that ybjN over-expression-related temperature rescue of the ts9 mutant may be due to down-regulation of metabolic activity and activation of stress response genes in the ts9 mutant.

Links

PubMed PMC3181261 Online version:10.1371/journal.pone.0025293

Keywords

Escherichia coli/cytology; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Gene Expression Regulation, Bacterial/genetics; Gene Expression Regulation, Bacterial/physiology; Mutation; Temperature

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ECOLI:YBJN

acts_upstream_of_or_within

GO:1902201: negative regulation of bacterial-type flagellum-dependent cell motility

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOK1:A1A992

GO:0051271: negative regulation of cellular component movement

ECO:0000315:

P

Figure 4B shows YbjN suppresses bacterial motility.

complete
CACAO 7123

ECOK1:A1A992

GO:0009242: colanic acid biosynthetic process

ECO:0000315:

P

Figure 5 shows negatively regulates colanic acid biosynthesis.

complete
CACAO 7142

ECOLW:E0IZI8

GO:0030092: regulation of flagellum assembly

ECO:0000315:

P

Figure 4C shows over-expression of ybjN negatively regulates flagellar gene expression.

complete
CACAO 7127

ECOLI:YBJN

involved_in

GO:1902208: regulation of bacterial-type flagellum assembly

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:YBJN

GO:1902209: negative regulation of bacterial-type flagellum assembly

ECO:0000315:

P

Figure 4C over-expression of ybjN negatively regulates flagellar gene expression.

complete
CACAO 7218

ECOLI:YBJN

GO:1900232: negative regulation of single-species biofilm formation on inanimate substrate

ECO:0000315:

P

Figure 6 shows YbjN is a negative regulator of biofilm formation.

complete
CACAO 7221

ECOLI:YBJN

GO:0009297: pilus assembly

ECO:0000315:

P

As shown in figure 3C, fimbriae are either malformed or not formed at all in cells with ybjN expression, whereas wild type cells produced substantial fimbriae.

complete
CACAO 7222

ECOLI:YBJN

GO:0030308: negative regulation of cell growth

ECO:0000315:

P

Figure 2 shows over-expression of ybjN leads to growth inhibition

complete
CACAO 7225

ECOLI:YBJN

involved_in

GO:1900232: negative regulation of single-species biofilm formation on inanimate substrate

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:YBJN

involved_in

GO:0030308: negative regulation of cell growth

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:YBJN

involved_in

GO:0009297: pilus assembly

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:YBJN

involved_in

GO:1902201: negative regulation of bacterial-type flagellum-dependent cell motility

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:YBJN

involved_in

GO:1902209: negative regulation of bacterial-type flagellum assembly

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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