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PMID:21976730

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Citation

Spedale, G, Meddens, CA, Koster, MJ, Ko, CW, van Hooff, SR, Holstege, FC, Timmers, HT and Pijnappel, WW (2012) Tight cooperation between Mot1p and NC2β in regulating genome-wide transcription, repression of transcription following heat shock induction and genetic interaction with SAGA. Nucleic Acids Res. 40:996-1008

Abstract

TATA-binding protein (TBP) is central to the regulation of eukaryotic transcription initiation. Recruitment of TBP to target genes can be positively regulated by one of two basal transcription factor complexes: SAGA or TFIID. Negative regulation of TBP promoter association can be performed by Mot1p or the NC2 complex. Recent evidence suggests that Mot1p, NC2 and TBP form a DNA-dependent protein complex. Here, we compare the functions of Mot1p and NC2βduring basal and activated transcription using the anchor-away technique for conditional nuclear depletion. Genome-wide expression analysis indicates that both proteins regulate a highly similar set of genes. Upregulated genes were enriched for SAGA occupancy, while downregulated genes preferred TFIID binding. Mot1p and NC2β depletion during heat shock resulted in failure to downregulate gene expression after initial activation, which was accompanied by increased TBP and RNA pol II promoter occupancies. Depletion of Mot1p or NC2β displayed preferential synthetic lethality with the TBP-interaction module of SAGA. Our results support the model that Mot1p and NC2β directly cooperate in vivo to regulate TBP function, and that they are involved in maintaining basal expression levels as well as in resetting gene expression after induction by stress.

Links

PubMed PMC3273795 Online version:10.1093/nar/gkr784

Keywords

Adenosine Triphosphatases/genetics; Adenosine Triphosphatases/metabolism; Cell Nucleus/metabolism; Gene Expression Regulation, Fungal; Genome, Fungal; Heat-Shock Proteins/metabolism; Heat-Shock Response; Promoter Regions, Genetic; RNA Polymerase II/metabolism; Repressor Proteins/genetics; Repressor Proteins/metabolism; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; TATA-Binding Protein Associated Factors/genetics; TATA-Binding Protein Associated Factors/metabolism; Trans-Activators/genetics; Transcription, Genetic

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:MOT1

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:MOT1

involved_in

GO:0045898: regulation of RNA polymerase II transcription preinitiation complex assembly

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:NCB2

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:NCB2

involved_in

GO:0045898: regulation of RNA polymerase II transcription preinitiation complex assembly

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:MOT1

part_of

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:MOT1

involved_in

GO:0045898: regulation of RNA polymerase II transcriptional preinitiation complex assembly

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

Notes

See also

References

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