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PMID:21969089

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Citation

Kim, JM, Woo, DH, Kim, SH, Lee, SY, Park, HY, Seok, HY, Chung, WS and Moon, YH (2012) Arabidopsis MKKK20 is involved in osmotic stress response via regulation of MPK6 activity. Plant Cell Rep. 31:217-24

Abstract

Plants have developed various regulatory pathways to adapt to environmental stresses. In this study, we identified Arabidopsis MKKK20 as a regulator in the response to osmotic stress. mkkk20 mutants were found to be sensitive to high concentration of salt and showed higher water loss rates than wild-type (WT) plants under dehydration conditions. In addition, mkkk20 mutants showed higher accumulation of superoxide, a reactive oxygen species (ROS), compared to WT plants under high salt condition. In contrast, transgenic plants overexpressing MKKK20 displayed tolerance to salt stress. MKKK20 transcripts were increased by the treatments with NaCl, mannitol, MV, sorbitol, and cold, suggesting that MKKK20 is involved in the response to osmotic, ROS, and cold stresses. In-gel kinase assay showed that MKKK20 regulates the activity of MPK6 under NaCl, cold, and H(2)O(2) treatments. Taken together, our results suggest that MKKK20 might be involved in the response to various abiotic stresses, especially osmotic stress, through its regulation of MPK6 activity.

Links

PubMed Online version:10.1007/s00299-011-1157-0

Keywords

Arabidopsis/drug effects; Arabidopsis/genetics; Arabidopsis/physiology; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Cold Temperature; Droughts; Gene Expression Regulation, Plant; Hydrogen Peroxide/pharmacology; Mannitol/pharmacology; Mitogen-Activated Protein Kinase Kinases/genetics; Mitogen-Activated Protein Kinase Kinases/metabolism; Mitogen-Activated Protein Kinases/genetics; Mitogen-Activated Protein Kinases/metabolism; Mutation; Osmotic Pressure; Phosphorylation; Plants, Genetically Modified; Reactive Oxygen Species/metabolism; Salt-Tolerance; Signal Transduction; Sodium Chloride/pharmacology; Sorbitol/pharmacology; Stress, Physiological; Superoxides/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:M3K20

acts_upstream_of_or_within

GO:0000302: response to reactive oxygen species

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:M3K20

acts_upstream_of_or_within

GO:0006468: protein phosphorylation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:M3K20

acts_upstream_of_or_within

GO:0009651: response to salt stress

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:M3K20

acts_upstream_of_or_within

GO:0009414: response to water deprivation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:M3K20

acts_upstream_of_or_within

GO:0009409: response to cold

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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