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PMID:21945443

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Citation

Zhang, X, Zhang, M, Takano, T and Liu, S (2011) Characterization of an AtCCX5 gene from Arabidopsis thaliana that involves in high-affinity K⁺ uptake and Na⁺ transport in yeast. Biochem. Biophys. Res. Commun. 414:96-100

Abstract

The gene for a putative cation calcium exchanger (CCX) from Arabidopsis thaliana, AtCCX5, was cloned and its function was analyzed in yeast. Green fluorescent protein-tagged AtCCX5 expressed in yeast was localized in the plasma membrane and nuclear periphery. The yeast transformants expressing AtCCX5 were created and their growth in the presence of various cations (K(+), Na(+), Ca(2+), Mg(2+), Fe(2+), Cu(2+), Co(2+), Cd(2+), Mn(2+), Ba(2+), Ni(2+), Zn(2+), and Li(+)) were analyzed. AtCCX5 expression was found to affect the response to K(+) and Na(+) in yeast. The AtCCX5 transformant also showed a little better growth to Zn(2+). The yeast mutant 9.3 expressing AtCCX5 restored growth of the mutant on medium with low K(+) (0.5mM), and also suppressed its Na(+) sensitivity. Ion uptake experiments showed that AtCCX5 mediated relatively high-affinity K(+) uptake and was also involved in Na(+) transport in yeast. Taken together, these findings suggest that the AtCCX5 is a novel transport protein involves in mediating high-affinity K(+) uptake and Na(+) transport in yeast.

Links

PubMed Online version:10.1016/j.bbrc.2011.09.030

Keywords

Amino Acid Sequence; Antiporters/genetics; Antiporters/metabolism; Arabidopsis/genetics; Arabidopsis/metabolism; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Cations, Monovalent/metabolism; Ion Transport; Molecular Sequence Data; Potassium/metabolism; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism; Sodium/metabolism; Suppression, Genetic

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:CCX5

located_in

GO:0034399: nuclear periphery

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:CCX5

acts_upstream_of_or_within

GO:0006814: sodium ion transport

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:CCX5

located_in

GO:0005886: plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:CCX5

enables

GO:0015079: potassium ion transmembrane transporter activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:CCX5

enables

GO:0015081: sodium ion transmembrane transporter activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:CCX5

acts_upstream_of_or_within

GO:0071805: potassium ion transmembrane transport

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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