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PMID:21916894

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Citation

Yang, Y, Jin, H, Chen, Y, Lin, W, Wang, C, Chen, Z, Han, N, Bian, H, Zhu, M and Wang, J (2012) A chloroplast envelope membrane protein containing a putative LrgB domain related to the control of bacterial death and lysis is required for chloroplast development in Arabidopsis thaliana. New Phytol. 193:81-95

Abstract

• A protein encoded by At1g32080 was consistently identified in proteomic studies of Arabidopsis chloroplast envelope membranes, but its function remained unclear. The protein, designated AtLrgB, may have evolved from a gene fusion of lrgA and lrgB. In bacteria, two homologous operons, lrgAB and cidAB, participate in an emerging mechanism to control cell death and lysis. • We aim to characterize AtLrgB using reverse genetics and cell biological and biochemical analysis. • AtLrgB is expressed in leaves, but not in roots. T-DNA insertion mutation of AtLrgB produced plants with interveinal chlorotic and premature necrotic leaves. Overexpression of full-length AtLrgB (or its LrgA and LrgB domains, separately), under the control of CaMV 35S promoter, produced plants exhibiting veinal chlorosis and delayed greening. At the end of light period, the T-DNA mutant had high starch and low sucrose contents in leaves, while the 35S:AtLrgB plants had low starch and high sucrose contents. Metabolite profiling revealed that AtLrgB appeared not to directly transport triose phosphate or hexose phosphates. In yeast cells, AtLrgB could augment nystatin-induced membrane permeability. • Our work indicates that AtLrgB is a new player in chloroplast development, carbon partitioning and leaf senescence, although its molecular mechanism remains to be established.

Links

PubMed Online version:10.1111/j.1469-8137.2011.03867.x

Keywords

Arabidopsis/drug effects; Arabidopsis/genetics; Arabidopsis/microbiology; Arabidopsis/ultrastructure; Arabidopsis Proteins/chemistry; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Bacteriolysis/drug effects; Cell Membrane Permeability/drug effects; Chloroplast Proteins/chemistry; Chloroplast Proteins/metabolism; Chloroplasts/drug effects; Chloroplasts/metabolism; Chloroplasts/ultrastructure; DNA, Bacterial/genetics; Evolution, Molecular; Gene Expression Regulation, Plant; Genes, Plant/genetics; Membrane Proteins/chemistry; Membrane Proteins/metabolism; Microbial Viability/drug effects; Mutagenesis, Insertional/genetics; Nystatin/pharmacology; Operon/genetics; Phenotype; Phosphates/metabolism; Plant Diseases/genetics; Plant Diseases/microbiology; Protein Structure, Tertiary; Protein Transport; Saccharomyces cerevisiae/cytology; Saccharomyces cerevisiae/drug effects; Saccharomyces cerevisiae/metabolism; Staphylococcus aureus/drug effects; Staphylococcus aureus/genetics; Starch/metabolism; Subcellular Fractions/drug effects; Subcellular Fractions/metabolism; Sucrose/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:PLGG1

located_in

GO:0009706: chloroplast inner membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:PLGG1

involved_in

GO:0009658: chloroplast organization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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