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PMID:21878498

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Citation

Richie, CT, Bembenek, JN, Chestnut, B, Furuta, T, Schumacher, JM, Wallenfang, M and Golden, A (2011) Protein phosphatase 5 is a negative regulator of separase function during cortical granule exocytosis in C. elegans. J. Cell. Sci. 124:2903-13

Abstract

Mutations in the Caenorhabditis elegans separase gene, sep-1, are embryonic lethal. Newly fertilized mutant embryos have defects in polar body extrusion, fail to undergo cortical granule exocytosis, and subsequently fail to complete cytokinesis. Chromosome nondisjunction during the meiotic divisions is readily apparent after depletion of sep-1 by RNAi treatment, but much less so in hypomorphic mutant embryos. To identify factors that influence the activity of separase in cortical granule exocytosis and cytokinesis, we carried out a genetic suppressor screen. A mutation in the protein phosphatase 5 (pph-5) gene was identified as an extragenic suppressor of sep-1. This mutation suppressed the phenotypes of hypomorphic separase mutants but not RNAi depleted animals. Depletion of pph-5 caused no phenotypes on its own, but was effective in restoring localization of mutant separase to vesicles and suppressing cortical granule exocytosis and cytokinesis phenotypes. The identification of PPH-5 as a suppressor of separase suggests that a new phospho-regulatory pathway plays an important role in regulating anaphase functions of separase.

Links

PubMed PMC3166036 Online version:10.1242/jcs.073379

Keywords

Alleles; Animals; Caenorhabditis elegans/enzymology; Caenorhabditis elegans/genetics; Caenorhabditis elegans/metabolism; Caenorhabditis elegans Proteins/genetics; Caenorhabditis elegans Proteins/metabolism; Cell Cycle Proteins/genetics; Cell Cycle Proteins/metabolism; Cytokinesis/genetics; Cytoplasmic Granules/genetics; Cytoplasmic Granules/metabolism; Endopeptidases/genetics; Endopeptidases/metabolism; Exocytosis/physiology; Mutation; Nuclear Proteins/biosynthesis; Nuclear Proteins/deficiency; Nuclear Proteins/genetics; Nuclear Proteins/metabolism; Phosphoprotein Phosphatases/biosynthesis; Phosphoprotein Phosphatases/deficiency; Phosphoprotein Phosphatases/genetics; Phosphoprotein Phosphatases/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CAEEL:E0R7L7

located_in

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:E0R7L7

involved_in

GO:0033365: protein localization to organelle

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CAEEL:E0R7L7

located_in

GO:0072686: mitotic spindle

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:E0R7L7

involved_in

GO:0045920: negative regulation of exocytosis

ECO:0000316: genetic interaction evidence used in manual assertion

WB:WBGene00004775

P

Seeded From UniProt

complete

CAEEL:SEP1

involved_in

GO:0000281: mitotic cytokinesis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CAEEL:SEP1

located_in

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:SEP1

located_in

GO:0060473: cortical granule

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:SEP1

involved_in

GO:0030703: eggshell formation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CAEEL:SEP1

located_in

GO:0072687: meiotic spindle

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:SEP1

located_in

GO:0072686: mitotic spindle

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:SEP1

located_in

GO:0005938: cell cortex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:SEP1

involved_in

GO:0051306: mitotic sister chromatid separation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CAEEL:SEP1

involved_in

GO:0061062: regulation of nematode larval development

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00092951

P

Seeded From UniProt

complete

CAEEL:SEP1

involved_in

GO:0060471: cortical granule exocytosis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CAEEL:SEP1

involved_in

GO:0048609: multicellular organismal reproductive process

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00092951

P

Seeded From UniProt

complete

CAEEL:SEP1

involved_in

GO:0040038: polar body extrusion after meiotic divisions

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CAEEL:SEP1

involved_in

GO:0040012: regulation of locomotion

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00092951

P

Seeded From UniProt

complete

CAEEL:PPP5

located_in

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:PPP5

involved_in

GO:0033365: protein localization to organelle

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CAEEL:PPP5

located_in

GO:0072686: mitotic spindle

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:PPP5

involved_in

GO:0045920: negative regulation of exocytosis

ECO:0000316: genetic interaction evidence used in manual assertion

WB:WBGene00004775

P

Seeded From UniProt

complete


See also

References

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