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PMID:21874009

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Citation

Yi, K, Unruh, JR, Deng, M, Slaughter, BD, Rubinstein, B and Li, R (2011) Dynamic maintenance of asymmetric meiotic spindle position through Arp2/3-complex-driven cytoplasmic streaming in mouse oocytes. Nat. Cell Biol. 13:1252-8

Abstract

Mature mammalian oocytes are poised for completing meiosis II (MII) on fertilization by positioning the spindle close to an actomyosin-rich cortical cap. Here, we show that the Arp2/3 complex localizes to the cortical cap in a Ran-GTPase-dependent manner and nucleates actin filaments in the cortical cap and a cytoplasmic actin network. Inhibition of Arp2/3 activity leads to rapid dissociation of the spindle from the cortex. Live-cell imaging and spatiotemporal image correlation spectroscopy analysis reveal that actin filaments flow continuously away from the Arp2/3-rich cortex, driving a cytoplasmic streaming expected to exert a net pushing force on the spindle towards the cortex. Arp2/3 inhibition not only diminishes this actin flow and cytoplasmic streaming but also enables a reverse streaming driven by myosin-II-based cortical contraction, moving the spindle away from the cortex. Thus, the asymmetric MII spindle position is dynamically maintained as a result of balanced forces governed by the Arp2/3 complex.

Links

PubMed Online version:10.1038/ncb2320

Keywords

Actin Cytoskeleton/drug effects; Actin Cytoskeleton/metabolism; Actin-Related Protein 2/genetics; Actin-Related Protein 2/metabolism; Actin-Related Protein 2-3 Complex/antagonists & inhibitors; Actin-Related Protein 2-3 Complex/genetics; Actin-Related Protein 2-3 Complex/metabolism; Actin-Related Protein 3/genetics; Actin-Related Protein 3/metabolism; Animals; Cells, Cultured; Cytoplasmic Streaming/drug effects; Female; Fourier Analysis; Heterocyclic Compounds with 4 or More Rings/pharmacology; Indoles/pharmacology; Kymography; Meiosis/drug effects; Mice; Microscopy, Confocal; Microscopy, Video; Mitotic Spindle Apparatus/drug effects; Mitotic Spindle Apparatus/metabolism; Morpholinos/metabolism; Myosin Type II/metabolism; Nocodazole/pharmacology; Oocytes/drug effects; Oocytes/metabolism; Protein Transport; Recombinant Fusion Proteins/metabolism; Time Factors; ran GTP-Binding Protein/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:ARP2

acts_upstream_of_or_within

GO:0030036: actin cytoskeleton organization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

  • occurs_in:(CL:0000023)

Seeded From UniProt

complete

MOUSE:ARP2

acts_upstream_of_or_within

GO:0016482: cytosolic transport

ECO:0000315: mutant phenotype evidence used in manual assertion

P

  • occurs_in:(CL:0000023)

Seeded From UniProt

complete

MOUSE:ARP2

located_in

GO:0030478: actin cap

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(CL:0000023)

Seeded From UniProt

complete

MOUSE:RAN

acts_upstream_of_or_within

GO:0030036: actin cytoskeleton organization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

  • occurs_in:(CL:0000023)

Seeded From UniProt

complete

MOUSE:RAN

acts_upstream_of_or_within

GO:0031503: protein-containing complex localization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:RAN

acts_upstream_of_or_within

GO:0034613: cellular protein localization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:WASL

acts_upstream_of_or_within

GO:0030036: actin cytoskeleton organization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

  • occurs_in:(CL:0000023)

Seeded From UniProt

complete

MOUSE:WASL

located_in

GO:0030478: actin cap

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(CL:0000023)

Seeded From UniProt

complete

MOUSE:WASL

acts_upstream_of_or_within

GO:0031503: protein-containing complex localization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:WASL

acts_upstream_of_or_within

GO:0051653: spindle localization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

  • occurs_in:(CL:0000023)

Seeded From UniProt

complete


See also

References

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