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PMID:21828100

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Citation

Moon, S, Cho, B, Min, SH, Lee, D and Chung, YD (2011) The THO complex is required for nucleolar integrity in Drosophila spermatocytes. Development 138:3835-45

Abstract

The THO complex is a conserved multisubunit protein complex that functions in the formation of export-competent messenger ribonucleoprotein (mRNP). Although the complex has been studied extensively at the single-cell level, its exact role at the multicellular organism level has been poorly understood. Here, we isolated a novel Drosophila male sterile mutant, garmcho (garm). Positional cloning indicated that garm encodes a subunit of the Drosophila THO complex, THOC5. Flies lacking THOC5 showed a meiotic arrest phenotype with severe nucleolar disruption in primary spermatocytes. A functional GFP-tagged fusion protein, THOC5-GFP, revealed a unique pattern of THOC5 localization near the nucleolus. The nucleolar distribution of a testis-specific TATA binding protein (TBP)-associated factor (tTAF), SA, which is required for the expression of genes responsible for sperm differentiation, was severely disrupted in mutant testes lacking THOC5. But THOC5 appeared to be largely dispensable for the expression and nuclear export of either tTAF target mRNAs or tTAF-independent mRNAs. Taken together, our study suggests that the Drosophila THO complex is necessary for proper spermatogenesis by contribution to the establishment or maintenance of nucleolar integrity rather than by nuclear mRNA export in spermatocytes.

Links

PubMed Online version:10.1242/dev.056945

Keywords

Animals; Blotting, Western; Cell Nucleolus/genetics; Cell Nucleolus/metabolism; Drosophila Proteins/genetics; Drosophila Proteins/metabolism; Drosophila melanogaster; Immunoprecipitation; In Situ Hybridization; Male; Nuclear Proteins/genetics; Nuclear Proteins/metabolism; Reverse Transcriptase Polymerase Chain Reaction; Spermatocytes/cytology; Spermatocytes/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:E2QCS8

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:M9PE28

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:THOC7

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:THOC7

part_of

GO:0032991: protein-containing complex

ECO:0000353: physical interaction evidence used in manual assertion

FB:FBgn0034939,FB:FBgn0037382

C

Seeded From UniProt

complete

DROME:Q960L4

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:Q9VNI8

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:Q9VNI8

part_of

GO:0032991: protein-containing complex

ECO:0000353: physical interaction evidence used in manual assertion

FB:FBgn0034939,FB:FBgn0035110

C

Seeded From UniProt

complete

DROME:Q9VQ76

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:THOC5

involved_in

GO:0007140: male meiotic nuclear division

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:THOC5

colocalizes_with

GO:0005730: nucleolus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:THOC5

colocalizes_with

GO:0000228: nuclear chromosome

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:THOC5

part_of

GO:0032991: protein-containing complex

ECO:0000353: physical interaction evidence used in manual assertion

FB:FBgn0035110,FB:FBgn0037382

C

Seeded From UniProt

complete


See also

References

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