PMID:21809413

Citation	Blom, H, Rönnlund, D, Scott, L, Spicarova, Z, Rantanen, V, Widengren, J, Aperia, A and Brismar, H (2012) Nearest neighbor analysis of dopamine D1 receptors and Na(+)-K(+)-ATPases in dendritic spines dissected by STED microscopy. Microsc. Res. Tech. 75:220-8
Abstract	Protein localization in dendritic spines is the focus of intense investigations within neuroscience. Applications of super-resolution microscopy to dissect nanoscale protein distributions, as shown in this work with dual-color STED, generate spatial correlation coefficients having quite small values. This means that colocalization analysis to some extent looses part of its correlative impact. In this study we thus introduced nearest neighbor analysis to quantify the spatial relations between two important proteins in neurons, the dopamine D1 receptor and Na(+),K(+)-ATPase. The analysis gave new information on how dense the D1 receptor and Na(+),K(+)-ATPase constituting nanoclusters are located both with respect to the homogenous (self to same) and the heterogeneous (same to other) topology. The STED dissected nanoscale topologies provide evidence for both a joint as well as a separated confinement of the D1 receptor and the Na(+),K(+)-ATPase in the postsynaptic areas of dendritic spines. This confined topology may have implications for generation of local sodium gradients and for structural and functional interactions modulating slow synaptic transmission processes.
Links	PubMed Online version:10.1002/jemt.21046
Keywords	Animals; Cells, Cultured; Dendritic Spines/chemistry; Image Interpretation, Computer-Assisted/instrumentation; Image Interpretation, Computer-Assisted/methods; Immunohistochemistry; Immunoprecipitation; Microscopy, Electron, Scanning Transmission; Microscopy, Fluorescence/instrumentation; Microscopy, Fluorescence/methods; Nanotechnology/methods; Neurons/chemistry; Neurons/metabolism; Rats; Rats, Sprague-Dawley; Receptors, Dopamine D1/analysis; Receptors, Dopamine D1/chemistry; Sodium-Potassium-Exchanging ATPase/analysis; Sodium-Potassium-Exchanging ATPase/chemistry
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
RAT:AT1A3	located_in	GO:0044326: dendritic spine neck	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
RAT:AT1A3	located_in	GO:0044327: dendritic spine head	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
RAT:AT1A3	enables	GO:0031748: D1 dopamine receptor binding	ECO:0000353: physical interaction evidence used in manual assertion	RGD:2518	F		Seeded From UniProt	complete
RAT:DRD1	located_in	GO:0044326: dendritic spine neck	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
RAT:DRD1	located_in	GO:0044327: dendritic spine head	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
RAT:DRD1	part_of	GO:0044326: dendritic spine neck	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
RAT:DRD1	part_of	GO:0044327: dendritic spine head	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
RAT:DRD1	enables	GO:0051117: ATPase binding	ECO:0000353: physical interaction evidence used in manual assertion	RGD:2169	F		Seeded From UniProt	complete

References

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