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PMID:21809413

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Citation

Blom, H, Rönnlund, D, Scott, L, Spicarova, Z, Rantanen, V, Widengren, J, Aperia, A and Brismar, H (2012) Nearest neighbor analysis of dopamine D1 receptors and Na(+)-K(+)-ATPases in dendritic spines dissected by STED microscopy. Microsc. Res. Tech. 75:220-8

Abstract

Protein localization in dendritic spines is the focus of intense investigations within neuroscience. Applications of super-resolution microscopy to dissect nanoscale protein distributions, as shown in this work with dual-color STED, generate spatial correlation coefficients having quite small values. This means that colocalization analysis to some extent looses part of its correlative impact. In this study we thus introduced nearest neighbor analysis to quantify the spatial relations between two important proteins in neurons, the dopamine D1 receptor and Na(+),K(+)-ATPase. The analysis gave new information on how dense the D1 receptor and Na(+),K(+)-ATPase constituting nanoclusters are located both with respect to the homogenous (self to same) and the heterogeneous (same to other) topology. The STED dissected nanoscale topologies provide evidence for both a joint as well as a separated confinement of the D1 receptor and the Na(+),K(+)-ATPase in the postsynaptic areas of dendritic spines. This confined topology may have implications for generation of local sodium gradients and for structural and functional interactions modulating slow synaptic transmission processes.

Links

PubMed Online version:10.1002/jemt.21046

Keywords

Animals; Cells, Cultured; Dendritic Spines/chemistry; Image Interpretation, Computer-Assisted/instrumentation; Image Interpretation, Computer-Assisted/methods; Immunohistochemistry; Immunoprecipitation; Microscopy, Electron, Scanning Transmission; Microscopy, Fluorescence/instrumentation; Microscopy, Fluorescence/methods; Nanotechnology/methods; Neurons/chemistry; Neurons/metabolism; Rats; Rats, Sprague-Dawley; Receptors, Dopamine D1/analysis; Receptors, Dopamine D1/chemistry; Sodium-Potassium-Exchanging ATPase/analysis; Sodium-Potassium-Exchanging ATPase/chemistry

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

RAT:AT1A3

located_in

GO:0044326: dendritic spine neck

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:AT1A3

located_in

GO:0044327: dendritic spine head

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:AT1A3

enables

GO:0031748: D1 dopamine receptor binding

ECO:0000353: physical interaction evidence used in manual assertion

RGD:2518

F

Seeded From UniProt

complete

RAT:DRD1

located_in

GO:0044326: dendritic spine neck

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:DRD1

located_in

GO:0044327: dendritic spine head

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:DRD1

part_of

GO:0044326: dendritic spine neck

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:DRD1

part_of

GO:0044327: dendritic spine head

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:DRD1

enables

GO:0051117: ATPase binding

ECO:0000353: physical interaction evidence used in manual assertion

RGD:2169

F

Seeded From UniProt

complete

Notes

See also

References

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