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PMID:21776080

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Citation

Russell, AB, Hood, RD, Bui, NK, LeRoux, M, Vollmer, W and Mougous, JD (2011) Type VI secretion delivers bacteriolytic effectors to target cells. Nature 475:343-7

Abstract

Peptidoglycan is the major structural constituent of the bacterial cell wall, forming a meshwork outside the cytoplasmic membrane that maintains cell shape and prevents lysis. In Gram-negative bacteria, peptidoglycan is located in the periplasm, where it is protected from exogenous lytic enzymes by the outer membrane. Here we show that the type VI secretion system of Pseudomonas aeruginosa breaches this barrier to deliver two effector proteins, Tse1 and Tse3, to the periplasm of recipient cells. In this compartment, the effectors hydrolyse peptidoglycan, thereby providing a fitness advantage for P. aeruginosa cells in competition with other bacteria. To protect itself from lysis by Tse1 and Tse3, P. aeruginosa uses specific periplasmically localized immunity proteins. The requirement for these immunity proteins depends on intercellular self-intoxication through an active type VI secretion system, indicating a mechanism for export whereby effectors do not access donor cell periplasm in transit.

Links

PubMed PMC3146020 Online version:10.1038/nature10244

Keywords

Amidohydrolases/chemistry; Amidohydrolases/genetics; Amidohydrolases/metabolism; Amino Acid Sequence; Bacterial Proteins/antagonists & inhibitors; Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Bacterial Proteins/secretion; Bacterial Secretion Systems; Bacterial Toxins/antagonists & inhibitors; Bacterial Toxins/metabolism; Bacteriolysis; Gram-Negative Bacteria/cytology; Gram-Negative Bacteria/metabolism; Hydrolysis; Microbial Interactions; Muramidase/chemistry; Muramidase/genetics; Muramidase/metabolism; Peptidoglycan/metabolism; Periplasm/metabolism; Pseudomonas aeruginosa/enzymology; Pseudomonas aeruginosa/genetics; Pseudomonas aeruginosa/growth & development; Pseudomonas aeruginosa/metabolism; Pseudomonas putida/growth & development; Pseudomonas putida/metabolism; Substrate Specificity

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

PSEAE:TSE3

enables

GO:0003796: lysozyme activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

PSEAE:TSE1

enables

GO:0004040: amidase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

PSEAE:Q9I2Q1

GO:0070011: peptidase activity, acting on L-amino acid peptides

ECO:0000315:

F

Figure 1-b shows that Tse-1 acts as a peptidase.

complete
CACAO 1980

PSEAE:Q9I2Q1

enables

GO:0070011: peptidase activity, acting on L-amino acid peptides

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete

PSEAE:Q9I2Q1

enables

GO:0004040: amidase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

PSEAE:Q9HYC5

GO:0003796: lysozyme activity

ECO:0000315:

F

Fig. 1D shows the relationship between Tse3 and lytic activity

complete
CACAO 2274

PSEAE:Q9HYC5

enables

GO:0003796: lysozyme activity

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete

PSEAE:Q9HYC5

enables

GO:0003796: lysozyme activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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