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PMID:21771930

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Citation

Stoppel, R, Lezhneva, L, Schwenkert, S, Torabi, S, Felder, S, Meierhoff, K, Westhoff, P and Meurer, J (2011) Recruitment of a ribosomal release factor for light- and stress-dependent regulation of petB transcript stability in Arabidopsis chloroplasts. Plant Cell 23:2680-95

Abstract

Land plant genomes encode four functional ribosomal peptide chain release factors (Prf) of eubacterial origin, two (PrfA and PrfB homologs) for each endosymbiotic organelle. Formerly, we have shown that the Arabidopsis thaliana chloroplast-localized PrfB homolog, PrfB1, is required not only for termination of translation but also for stabilization of UGA stop codon-containing chloroplast transcripts. A previously undiscovered PrfB-like protein, PrfB3, is localized to the chloroplast stroma in a petB RNA-containing complex and found only in vascular plants. Highly conserved positions of introns unequivocally indicate that PrfB3 arose from a duplication of PrfB1. Notably, PrfB3 is lacking the two most important tripeptide motifs characteristic for all eubacterial and organellar PrfB homologs described so far: the stop codon recognition motif SPF and the catalytic center GGQ for peptidyl-tRNA hydrolysis. Complementation studies, as well as functional and molecular analyses of two allelic mutations in Arabidopsis, both of which lead to a specific deficiency of the cytochrome b₆f complex, revealed that PrfB3 is essentially required for photoautotrophic growth. Plastid transcript, polysome, and translation analyses indicate that PrfB3 has been recruited in vascular plants for light- and stress-dependent regulation of stability of 3' processed petB transcripts to adjust cytochrome b₆ levels.

Links

PubMed PMC3226201 Online version:10.1105/tpc.111.085324

Keywords

Amino Acid Sequence; Arabidopsis/genetics; Arabidopsis/metabolism; Arabidopsis Proteins/classification; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Chloroplasts/genetics; Chloroplasts/metabolism; Chloroplasts/ultrastructure; Codon, Terminator/metabolism; Cytochrome b6f Complex/genetics; Cytochrome b6f Complex/metabolism; Electron Transport; Gene Expression Regulation, Plant; Light; Molecular Sequence Data; Multigene Family; Mutation; Phylogeny; Plant Proteins/genetics; Plant Proteins/metabolism; Protein Isoforms/genetics; Protein Isoforms/metabolism; RNA Stability; RNA-Binding Proteins/classification; RNA-Binding Proteins/genetics; RNA-Binding Proteins/metabolism; Ribosomal Proteins/classification; Ribosomal Proteins/genetics; Ribosomal Proteins/metabolism; Sequence Alignment; Stress, Physiological

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:PRFB3

located_in

GO:0009507: chloroplast

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:PRFB3

located_in

GO:0009570: chloroplast stroma

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:PRFB3

involved_in

GO:0009658: chloroplast organization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:PRFB3

enables

GO:0003730: mRNA 3'-UTR binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:PRFB3

involved_in

GO:0043488: regulation of mRNA stability

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:PRFB3

enables

GO:0043565: sequence-specific DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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