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PMID:21752937

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Citation

Casper, AL, Baxter, K and Van Doren, M (2011) no child left behind encodes a novel chromatin factor required for germline stem cell maintenance in males but not females. Development 138:3357-66

Abstract

Male and female germ cells follow distinct developmental paths with respect to germline stem cell (GSC) production and the types of differentiated progeny they produce (sperm versus egg). An essential aspect of germline development is how sexual identity is used to differentially regulate the male and female germ cell genomes to allow for these distinct outcomes. Here, we identify a gene, no child left behind (nclb), that plays very different roles in the male versus female germline in Drosophila. In particular, nclb is required for GSC maintenance in males, but not in females. Male GSCs mutant for nclb are rapidly lost from the niche, and begin to differentiate but cannot complete spermatogenesis. We further find that nclb encodes a member of a new family of conserved chromatin-associated proteins. NCLB interacts with chromatin in a specific manner and is associated with sites of active transcription. Thus, NCLB appears to be a novel chromatin regulator that exhibits very different effects on the male and female germ cell genomes.

Links

PubMed PMC3143560 Online version:10.1242/dev.067942

Keywords

Animals; Chromatin/metabolism; Chromosomal Proteins, Non-Histone/genetics; Chromosomal Proteins, Non-Histone/metabolism; Drosophila Proteins/genetics; Drosophila Proteins/metabolism; Drosophila melanogaster/cytology; Drosophila melanogaster/embryology; Drosophila melanogaster/metabolism; Epigenesis, Genetic; Female; Gene Expression Regulation, Developmental; Male; Mutation; Oogenesis; Ovum/cytology; Ovum/metabolism; Spermatogenesis; Spermatozoa/cytology; Spermatozoa/metabolism; Stem Cells/cytology; Stem Cells/metabolism; Transcription, Genetic

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:PWP1

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:PWP1

colocalizes_with

GO:0035327: transcriptionally active chromatin

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:PWP1

involved_in

GO:0030723: ovarian fusome organization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:PWP1

involved_in

GO:0030707: ovarian follicle cell development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:PWP1

involved_in

GO:0010468: regulation of gene expression

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:PWP1

involved_in

GO:0007281: germ cell development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:PWP1

involved_in

GO:0008584: male gonad development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:PWP1

enables

GO:0031490: chromatin DNA binding

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete

DROME:PWP1

NOT|involved_in

GO:0036099: female germ-line stem cell population maintenance

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:PWP1

involved_in

GO:0036098: male germ-line stem cell population maintenance

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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