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PMID:21742768

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Citation

Nishida, S, Tsuzuki, C, Kato, A, Aisu, A, Yoshida, J and Mizuno, T (2011) AtIRT1, the primary iron uptake transporter in the root, mediates excess nickel accumulation in Arabidopsis thaliana. Plant Cell Physiol. 52:1433-42

Abstract

Nickel (Ni) is an essential nutrient for plants, but excessive amounts can be toxic. Ni competes with iron (Fe) in vivo, raising the possibility that Ni is competitively taken up via the Fe uptake system in plants. Here, we show evidence that AtIRT1, the primary Fe(2+) uptake transporter in the root, mediates Ni accumulation in Arabidopsis thaliana. In hydroponic cultures, excess Ni exposure increased Fe accumulation and the relative transcription level of AtIRT1 in roots, indicating that excess Ni induces AtIRT1 expression in roots. An Fe-deficient treatment increased Ni accumulation in plants, suggesting that excess Ni was absorbed via the Fe uptake system, which was induced by Fe starvation. Moreover, Ni accumulation under Fe-deficient conditions was markedly lower in AtIRT1-defective mutants than in the wild-type, Col-0. Furthermore, AtIRT1 showed Ni(2+) uptake activity in a yeast expression system. These data demonstrate that AtIRT1 transports Ni(2+) in roots, and strongly suggest that Ni accumulation is further accelerated by AtIRT1 that is expressed in response to excess Ni.

Links

PubMed Online version:10.1093/pcp/pcr089

Keywords

Adaptation, Physiological/drug effects; Adaptation, Physiological/genetics; Arabidopsis/drug effects; Arabidopsis/genetics; Arabidopsis/metabolism; Arabidopsis/physiology; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Cation Transport Proteins/genetics; Cation Transport Proteins/metabolism; Gene Expression Regulation, Plant/drug effects; Hydroponics; Iron/metabolism; Iron/pharmacology; Mutation/genetics; Nickel/metabolism; Nickel/toxicity; Phenotype; Plant Roots/drug effects; Plant Roots/genetics; Plant Roots/metabolism; Saccharomyces cerevisiae/cytology; Saccharomyces cerevisiae/drug effects; Saccharomyces cerevisiae/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:IRT1

acts_upstream_of_or_within

GO:0015675: nickel cation transport

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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